榧犵柅鑰跺皵妫弻榧犵柅鏉嗚弻PCR妫€娴嬭瘯鍓傜洅浣跨敤鏂规硶:

銆€銆€娉?鎵€鏈夎瘯鍓備娇鐢ㄥ墠闇€瀹屽叏瑙ｅ喕,娣峰悎鍧囧寑,6,000rpm绂诲績鏁扮鍚庝娇鐢ㄣ€侟/span>

銆€銆€1. 鏍锋湰澶勭悊(鏍锋湰澶勭悊鍖?

銆€銆€寰呮鏍锋湰鐨勬牳閰告彁鍙栧彲閲囩敤鐥呮瘨RNA鎻愬彇璇曞墏鐩掓垨鑷姩鍖栨牳閰告彁鍙栦华绛?鍏蜂綋鎻愬彇鏂规硶璇峰弬鐓х浉鍏宠鏄庝功銆傞槼鎬ц川鎺у搧鍙婇槾鎬ц川鎺у搧鏃犻渶鎻愬彇,鍙洿鎺ヤ娇鐢ㄣ€侟/span>

銆€銆€2. 鎵╁璇曞墏鍑嗗(PCR鍓嶅噯澶囧尯)

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銆€銆€缁勫垎姣忓ご浠界敤閲廎MD RT-PCR鍙嶅簲娑?9 渭lRT-PCR閰躲€侟/span>

銆€銆€1 .灏嗘墍鏈塒CR鍙嶅簲绠′簬6,000rpm绂诲績30s,杞Щ鑷虫牱鏈鐞嗗尯銆侟/span>

銆€銆€2.鍔犳牱(鏍锋湰澶勭悊鍖?

銆€銆€3.鍦ㄤ笂杩扮殑PCR鍙嶅簲绠′腑鍒嗗埆鍔犲叆寰呮鏍锋湰RNA鎻愬彇鐗┿€侀槾鎬ц川鎺у搧鍜岄槼鎬ц川鎺у搧鍚? 渭l,鐩栫揣绠＄洊,浜?,000rpm绂诲績10s,杞Щ鑷虫墿澧炲尯銆侟/span>