IL-1伪 灏忛紶鐧戒粙绱?伪ELISA妫€娴嬭瘯鍓傜洅璇曞墏閰嶅埗锛欬/span>

1銆侀叾鑱旀澘锛圓ssay plate 锛夛細涓€鍧楋紙96瀛旀垨鑰?8瀛旓級銆侟/span>

2銆佹爣鍑嗗搧锛圫tandard锛夛細2鐡讹紙鍐诲共鍝侊級銆侟/span>

3銆佹牱鍝佺█閲婃恫锛圫ample Diluent锛夛細1脳20ml/鐡躲€侟/span>

4銆佹爣璁版姉浣撶█閲婃恫锛圔iotin-antibody Diluent锛夛細1脳10ml/鐡躲€侟/span>

5銆佽荆鏍硅繃姘у寲鐗╅叾鏍囪浜插拰绱犵█閲婃恫 锛圚RP-avidin Diluent锛夛細1脳10ml/鐡躲€侟/span>

6銆佹爣璁版姉浣擄紙Biotin-antibody锛夛細1脳120渭l/鐡讹紙1锛?00锛堻/span>

7銆佽荆鏍硅繃姘у寲鐗╅叾鏍囪浜插拰绱狅紙HRP-avidin锛夛細1脳120渭l/鐡讹紙1锛?00锛堻/span>

8銆佸簳鐗╂憾娑诧紙TMB Substrate锛夛細1脳10ml/鐡躲€侟/span>

9銆佹祿娲楁钉娑诧紙Wash Buffer锛夛細1脳20ml/鐡讹紝浣跨敤鏃舵瘡鐡剁敤钂搁姘寸█閲?5鍊嶃€侟/span>

10銆佺粓姝㈡恫锛圫top Solution锛夛細1脳10ml/鐡讹紙2N H2SO4锛夈€侟/span>