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3銆佸墿涓嬬殑缁勭粐鍐嶅姞鍏?锝?mL閰舵秷鍖栨恫锛屾贩鎮?0s锛岀疆37鈩冩秷鍖?0 min鍚庯紝鎸変笂杩版柟娉曟敹闆嗕笂娓呭苟缁堟娑堝寲鍚?鈩冩斁缃紝閲嶅姝ゆ楠?-3娆★紝鐩磋嚦缁勭粐瀹屽叏琚秷鍖栵紱

4銆佺敤200鐩笉閿堥挗绛涚綉杩囨护缁嗚優娑堝寲娑诧紝1200r/min 绂诲績10min锛屽純鍘讳笂娓咃紝娌夋穩缁嗚優鐢ㄥ惈鏈?0锛 FBS DMEM/F12鍩瑰吇鍩烘贩鎮紝鎺ョ浜?5cm2鍩瑰吇鐡讹紝鏀剧疆浜?7鈩 锛?锛匔O2 鍩瑰吇绠变腑鍩瑰吇锛汓/span>

5銆佸樊閫熻创澹?h鍚庯紝鍚稿嚭鍩瑰吇鍩猴紝鎸夊疄楠岄渶瑕佹帴绉嶄簬6瀛旀澘涓户缁煿鍏伙紱

浜屻€佸厤鐤崸鍏夐壌瀹氾細

1銆佸緟蹇冩埧鑲岀粏鑳炵敓闀胯嚦80%铻嶅悎鏃讹紝寮冨幓鍩瑰吇鍩猴紝鐢ㄦ俯鑲茬殑PBS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庣敤4%澶氳仛鐢查啗鍦ㄥ娓╂潯浠朵笅鍥哄畾缁嗚優15min锛汓/span>

2銆丳BS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庡湪4鈩冩潯浠朵笅锛岀敤0.1锛匱riton X-100閫忚啘15min锛汓/span>

3銆丳BS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庡湪瀹ゆ俯鏉′欢涓嬶紝鐢?% BSA灏侀棴缁嗚優30min锛汓/span>

4銆佹寜1: 100鐨勬瘮渚嬬█閲娢?actin涓€鎶楋紙Abbioscience鍏徃锛夛紝鐒跺悗灏嗗叾鏀惧湪4鈩冪涓鑲茬粏鑳炶繃澶滐紱

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6銆佺敤PBS鍐叉礂3娆★紝姣忔10min锛屾渶鍚庡湪鍊掔疆鑽у厜鏄惧井闀滀笅瑙傚療鍥惧儚骞舵媿鐓с€侟/span>

MFAP5(Microfibrillar-associated protein 5) ELISA KitRat Trpv1(Transient receptor potential cation channel subfamily V member 1) ELISA KitO-鍙斾竵鍩?L-閰皑閰哥粍缁囧熀璐ㄩ噾灞炶泲鐧介叾锛圡MP-9锛夋椿鎬ц崸鍏夊畾閲忔娴嬭瘯鍓傜洅

WDR45 ELISA KitRat Rrm2(Ribonucleoside-diphosphate reductase subunit M2) ELISA KitO-鍙斾竵鍩?L-閰皑閰镐綋娑插熀璐ㄩ噾灞炶泲鐧介叾锛圡MP-9锛夋椿鎬ц崸鍏夊畾閲忔娴嬭瘯鍓傜洅

WDR46 ELISA KitRat GT(Gastrin) ELISA Kit娌硅兒缁嗚優鍩鸿川閲戝睘铔嬬櫧閰讹紙MMP-9锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

ZP3(Zona pellucida sperm-binding protein 3) ELISA KitRat Dio1(Type I iodothyronine deiodinase) ELISA Kit娌硅兒缁勭粐鍩鸿川閲戝睘铔嬬櫧閰讹紙MMP-9锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

WAPL/WAPAL ELISA KitRat Dio3(Type III iodothyronine deiodinase) ELISA Kit(2R,3R)-(-)-1,4-鍙?浜岃嫰鍩鸿啨鍩?-2,3-O-寮備簹涓欏熀-2,3-涓佷簩閱囦綋娑插熀璐ㄩ噾灞炶泲鐧介叾锛圡MP-9锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

VWA2 ELISA KitRat Plg(Plasminogen) ELISA Kit(2R,3R)-(-)-1,4-鍙?浜岃嫰鍩鸿啨鍩?-2,3-O-寮備簹涓欏熀-2,3-涓佷簩閱囩粏鑳炲熀璐ㄩ噾灞炶泲鐧介叾锛圡MP-10锛夋椿鎬ц崸鍏夊畾閲忔娴嬭瘯鍓傜洅

WDR16 ELISA KitRat Fabp3(Fatty acid-binding protein, heart) ELISA Kit澶氳偨璇曞墏TCTU缁勭粐鍩鸿川閲戝睘铔嬬櫧閰讹紙MMP-10锛夋椿鎬ц崸鍏夊畾閲忔娴嬭瘯鍓傜洅

WDFY3 ELISA KitRat Foxc2(Forkhead box protein C2) ELISA Kit澶氳偨璇曞墏TCTU浣撴恫鍩鸿川閲戝睘铔嬬櫧閰讹紙MMP-10锛夋椿鎬ц崸鍏夊畾閲忔娴嬭瘯鍓傜洅

P3X63Ag8.653(楠ㄩ珦鐦ょ粏鑳?PROZ(Vitamin K-dependent protein Z) ELISA KitBlood Group Antigen Precursor 琛€鍨嬫姉鍘熷墠浣撹泲鐧芥姉浣?-纰樺悺鍜兎-1-缇ч吀鍙斾竵閰?锛?-浜岄厷杩涘彛/鍥戒骇RAB3A ras鐧屽洜瀹舵棌Rab3a鎶椾綋鍚噺锛氭鏌?/span>

MTHFD1 ELISA KitBlood Group Kell Antigen/CD238 鍑皵琛€鍨嬬硸铔嬬櫧CD238鎶椾綋2,4,6-涓夋捍鑻厷鐜垔鍡繘鍙?鍥戒骇Rab3A+Rab3B+Rab3C+Rab3D ras鐧屽洜瀹舵棌Rab3A--Rab3D鎶椾綋鍚噺锛氬惈閲忔祴瀹欬/span>

MTHFD2(Methylenetetrahydrofolate dehydrogenase 2) ELISA KitBlood Group Lewis a 琛€鍨婰ewis a鎶椾綋2,4,6-涓夋捍鑻厷澶氬反杩涘彛/鍥戒骇RBP4 瑙嗛粍閱囩粨鍚堣泲鐧?鎶椾綋鍚噺锛氬惈閲忔祴瀹欬/span>

CDK5(Cyclin-dependent kinase 5) ELISA KitDARC/CD234 Duffy琛€鍨嬭秼鍖栧洜瀛愬彈浣撴姉浣撶墰纾虹尓鍘绘哀鑳嗛吀鑺偅閰歌繘鍙?鍥戒骇REG1A 鑳板矝缁嗚優鍐嶇敓鍥犲瓙ICRF鎶椾綋鍚噺锛歎V娉曟憾鍑哄害妫€鏌?/span>

MTHFD1L ELISA KitGalactosidase alpha 伪-鍗婁钩绯栬嫹閰舵姉浣?,4,6-涓夌敳鍩鸿嫰鑳虹鍞戣繘鍙?鍥戒骇Renin /琛€绠＄揣寮犲舰鎴愰叾Ren1鎶椾綋鍚噺锛氬惈閲忔祴瀹欬/span>

MTHFS ELISA KitAnkyrin erythroid 绾㈢粏鑳炶泲鐧紸nk1鎶椾綋2,4,6-涓夌敳鍩鸿嫰鑳鸿仈鍙岄叝杩涘彛/鍥戒骇RSPO1 鍒嗘硨鎬ц泲鐧絉SPO1鎶椾綋鍚噺锛氬惈閲忔祴瀹欬/span>

MTHFD1L ELISA KitPPM1F 閽欒皟铔嬬櫧渚濊禆鎬ц泲鐧芥縺閰剁７閰搁叾鎶椾綋姘熺〖閰歌灪鍐呴叝杩涘彛/鍥戒骇SERPIN A11 涓濇皑閰歌泲鐧介叾鎶戝埗鍓侫11鎶椾綋鍚噺锛氭鏌?/span>

MTHFS ELISA KitRIP3 鍙椾綋缁撳悎涓濇皑閰歌嫃姘ㄩ吀婵€閰?鎶椾綋鍙嶅紡,鍙嶅紡-閲戝悎娆㈤唶鍖栬繘鍙?鍥戒骇SERPING1/C1 Inactivator 閰叾鎶戝埗铔嬬櫧C1IN鎶椾綋鍚噺锛氬惈閲忔祴瀹欬/span>