NIH:OVCAR-3(鍗靛发鐧岀粏鑳?鐜拌揣渚涘簲锛屼环鏍间紭鎯犮€佸疄楠屾晥鏋滃ソ锛屾垜鍙镐负鎮ㄦ彁渚涘厤璐逛唬娴嬫湇鍔★紝鍚屾椂鎻愪緵鏈€鏂颁环鏍笺€佽鏄庝功銆佽鏍笺€佺敤閫斻€佸疄楠屽師鐞嗙瓑鐩稿叧鎿嶄綔璇存槑銆侟/span>

浜у搧鍚嶇О	NIH:OVCAR-3(鍗靛发鐧岀粏鑳?
浜у搧绠€绉?/span>	NIH:OVCAR-3
璐у彿	GOY-X6387

缁嗚優鐨勭绫伙細

绗楃锛欬/span>

鏄喕瀛樹骇鍝侊紝鐢辨恫姘洿鎺ユ嬁鍑猴紝骞茶繍杈撶粰瀹㈡埛銆備竴鑸笉鎺ㄨ崘杩欑锛屼篃杈冨皯浣跨敤杩欑鏂瑰紡锛屽洜涓哄鑻忔墜娉曞浜庣粏鑳炵姸鎬佸奖鍝嶈緝澶э紝涓€鏃︾粏鑳炵姸鎬佷笉濂斤紝寰堥毦璇存槸缁嗚優鑷韩涓嶈锛岃繕鏄鑻忔病鎿嶄綔濂斤紱鍙﹀娓╁害瀵圭粏鑳炪€佸熀鍥犲姛鑳界姸鎬佸奖鍝嶅緢澶э紝涔熶笉鏄粏鑳炲偍瀛樼殑zui濂芥柟寮忥紝蹇€掓椂闂翠篃寰堥毦鎺у埗锛屽绉嶅洜绱犲奖鍝嶄骇鍝佺殑璐ㄩ噺锛涘共杩愯緭闇€瑕侀澶栨坊鍔?00鍏冪殑杩愯垂(椤轰赴)銆侟/span>

绗簩绉嶏細

鏄垜浠鑻忓ソ缁嗚優锛孅/span>QC閫氳繃鍚庯紝T-25鐏屾弧鍩瑰吇鍩哄父娓╄繍杈撶粰瀹㈡埛锛屾帓闄ゅ鑻忛€犳垚褰卞搷锛屽父娓╄繍杈撲篃瀵圭粏鑳炲奖鍝嶄篃涓嶅ぇ锛屽彧闇€鍔?5鍏冭繍璐?椤轰赴)銆侟/span>

璐ㄩ噺淇濊瘉锛氭垜浠殑缁嗚優鏍潵婧愪簬ATCC銆丒CACC銆丏SMZ銆丣CRB绛夛紝璐拱鍒拌揣鍚庯紝鐢辨垜浠疄楠屽鎵╁銆佸喕瀛橈紝鍐诲瓨鐨勪骇鍝佸叏閮ㄧ粡杩嘠C妫€娴嬶紝100%杩涘彛鏉ユ簮锛?00%淇濊瘉5浠ｄ互鍐咃紝娲诲姏>95%锛屾棤缁嗚弻銆佺湡鑿屻€佹敮鍘熶綋姹℃煋銆傜殑鍐诲瓨鏄粏鑳炲煿鍏荤殑鍩烘湰鎿嶄綔锛屼笉鍚岀殑瀹為獙瀹ら噰鐢ㄧ殑鏂规硶鐣ユ湁宸紓锛屼絾鏄兘浼氶伒寰參鍐婚€熸憾鐨勫畻鏃ㄣ€侟/span>

銆愬煿鍏绘寚鍗椼€慄/span>

瀵逛簬璐村缁嗚優锛屼紶浠ｅ彲鍙傝€冧互涓嬫柟娉曪細

1. 寮冨幓鍩瑰吇涓婃竻锛岀敤涓嶅惈閽欍€侀晛绂诲瓙鐨凱BS娑︽礂缁嗚優1-2娆°€侟/span>

2. 鍔?ml娑堝寲娑?0.25%Trypsin-0.53mM EDTA)浜庡煿鍏荤摱涓紝缃簬37鈩冨煿鍏荤涓秷鍖?-2鍒嗛挓锛岀劧鍚庡湪鏄惧井闀滀笅瑙傚療鈪″瀷鑲烘场涓婄毊缁嗚優|缁嗚優娑堝寲鎯呭喌锛岃嫢缁嗚優澶ч儴鍒嗗彉鍦嗗苟鑴辫惤锛岃繀閫熸嬁鍥炴搷浣滃彴锛岃交鏁插嚑涓嬪煿鍏荤摱鍚庡姞灏戦噺鍩瑰吇鍩虹粓姝㈡秷鍖栥€侟/span>

3. 鎸?-8ml/鐡惰ˉ鍔犲煿鍏诲熀锛岃交杞绘墦鍖€鍚庡惛鍑猴紝鍦?000RPM鏉′欢涓嬬蹇?鍒嗛挓锛屽純鍘讳笂娓呮恫锛岃ˉ鍔?-2mL鍩瑰吇娑插悗鍚瑰寑銆侟/span>

4. 灏嗙粏鑳炴偓娑叉寜1锛?鍒?锛?鐨勬瘮渚嬪垎鍒版柊鐨勫惈8ml鍩瑰吇鍩虹殑鏂扮毧涓垨鑰呯摱涓€侟/span>

銆愮粏鑳炲喕瀛樸€慄/span>

浜у搧鍚嶇О寰呯粏鑳炵敓闀跨姸鎬佽壇濂芥椂锛屽彲杩涜293/HEK-293缁嗚優|缁嗚優鍐诲瓨銆傝创澹佺粏鑳炲喕瀛樻椂锛屽純鍘诲煿鍏诲熀鍚庡姞鍏ュ皯閲忚儼閰讹紝缁嗚優鍙樺渾鑴辫惤鍚庯紝鍔犲叆绾?ml鍚娓呯殑鍩瑰吇鍩哄悗鍔犲叆鍐诲瓨绠′腑锛屽啀娣诲姞10%DMSO鍚庤繘琛屽喕瀛樸€傛偓娴粏鑳炲喕瀛樻椂锛屽簲灏嗙粏鑳炴敹闆嗭紝1000RPM鏉′欢涓嬬蹇?鍒嗛挓锛屽皯閲忎繚瀛樹笂娓呮恫(闃叉缁嗚優鍚歌蛋)锛屽姞鍏ラ儴鍒嗘柊椴滃煿鍏诲熀锛屽姞鍏ュ埌鍐诲瓨绠′腑锛屽湪鍐诲瓨绠′腑鍔犲叆10%DMSO鍚庤繘琛屽喕瀛樸€侟/span>

銆愬鑻忕殑鍘熷垯銆慄/span>

蹇€熻瀺鍖栵細蹇呴』灏嗗喕瀛樺湪-196鈩冩恫姘腑鐨勭粏鑳炲揩閫熻瀺鍖栬嚦37鈩冿紝浣跨粏鑳炲鍐诲瓨鏃剁殑鏅惰繀閫熻瀺鍖栵紝閬垮厤鏅剁紦鎱㈣瀺鍖栨椂杩涘叆缁嗚優褰㈡垚鍐嶇粨鏅讹紝瀵圭粏鑳為€犳垚鎹熷銆傚鏃︾粏鑳炲簱鍏ㄥ浗鍚勫湴鍧囧彲鍙戣揣锛屽叏鍥界粺涓€浠锋牸锛屾湰鍏徃鍑哄敭鐨勬墍鏈夌粏鑳炰粎渚涚鐮斾娇鐢ㄣ€侟/span>

OLFM2 ELISA KitSAP62 鍓帴浣撶浉鍏宠泲鐧?2鎶椾綋鍗曞畞閰稿墤鍙堕緳琛€C杩涘彛/鍥戒骇Hepatitis C Virus RNA-directed RNA polymerase 鍨嬭倽鐐庣梾NS4B锛?5kDa锛夋姉浣撳惈閲忥細HPLC鈮?8%

OLFM3 ELISA KitERR-alpha 闆屾縺绱犲彈浣撶浉鍏宠泲鐧轿辨姉浣撳崟瀹侀吀/涓瑰畞閰?闉ｉ吀杈涘し鑴傝繘鍙?鍥戒骇SORBS2/ArgBP2 姘ㄩ吀缁撳悎铔嬬櫧2鎶椾綋鍚噺锛欻PLC鈮?8%

OLIG2(Oligodendrocyte transcription factor 2) ELISA KitDCHS1 閽欑矘铔嬬櫧19鎶椾綋6-缇熷熀-2,4,5-涓夋皑鍩哄槯鍟朵箤鑽唴鑴傝繘鍙?鍥戒骇BNP 鑴戦挔/鍒╅挔鑲芥姉浣撳惈閲忥細HPLC鈮?8%

OMA1 ELISA KitAXL/UFO 绮橀檮鐩稿叧婵€閰舵姉浣?-缇熷熀-2,4,5-涓夋皑鍩哄槯鍟舵紗榛?闈炵憻/绱搯)杩涘彛/鍥戒骇p70 S6 kinase beta 鏍哥硸浣撹泲鐧絊6婵€閰?鎶椾綋鍚噺锛欻PLC鈮?8%

ODF2L ELISA KitDelta Catenin 未杩炵幆铔嬬櫧/未杩炵幆绱?未閾炬帴绱犳姉浣擄紙未-catenin锛?-缇熷熀-2,4,5-涓夋皑鍩哄槯鍟惰嫤鐜勫弬鑻封厾A杩涘彛/鍥戒骇CD20 CD20鎶椾綋鍚噺锛欻PLC鈮?8%

OMCG1 ELISA KitOCIAD1 鍗靛发鐧屽厤鐤弽搴旀姉鍘熸姉浣?-缇熷熀-2,4,5-涓夋皑鍩哄槯鍟惰嫤鐜勫弬鑻封厾B杩涘彛/鍥戒骇Complement C3 beta chain 琛ヤ綋C3b鎶椾綋鍚噺锛欻PLC鈮?8%

OCTN2 ELISA KitPAGE4/GAGE9 鍓嶅垪鑵虹檶鐩稿叧铔嬬櫧4鎶椾綋纭厜姘旇姦鑽熻嫹杩涘彛/鍥戒骇C3a/C3a anaphylatoxin 琛ヤ綋C3a杩囨晱鎶椾綋鍚噺锛欻PLC鈮?8%

OCRL ELISA KitPMPCB 绾跨矑浣撳鑲芥按瑙ｉ叾尾鎶椾綋纭厜姘旂櫧鏈唴閰厾杩涘彛/鍥戒骇Complement C3b alpha' chain 琛ヤ綋C3b-伪閾炬姉浣撳惈閲忥細HPLC鈮?8%

NIH:OVCAR-3(鍗靛发鐧岀粏鑳?ZNRF2(Zinc and ring finger 2) ELISA KitRat NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) ELISA KitN-缇熷熀鐞ョ弨閰颁簹鑳虹粏鑳濶ADPH缁嗚優鑹茬礌C杩樺師閰讹紙NADPH Cytochrome C Reductase锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

ZPBP ELISA KitRat PDGFB(Platelet-derived growth factor subunit B) ELISA KitN-缇熷熀鐞ョ弨閰颁簹鑳虹粍缁嘚ADPH缁嗚優鑹茬礌C杩樺師閰讹紙NADPH Cytochrome C Reductase锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

ZO-2 ELISA KitRat IL-7(Interleukin-7) ELISA KitN-鑻勬哀缇板熀-L-璋锋皑閰哥粏鑳濶ADPH缁嗚優鑹茬礌C杩樺師閰讹紙NADPH Cytochrome C Reductase锛夋椿鎬ч珮璐ㄦ晱鎰熸瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

ZNF740 ELISA KitRat FGF23(Fibroblast growth factor 23) ELISA KitN-鑻勬哀缇板熀-L-璋锋皑閰哥粍缁嘚ADPH缁嗚優鑹茬礌C杩樺師閰讹紙NADPH Cytochrome C Reductase锛夋椿鎬ч珮璐ㄦ晱鎰熸瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

ZNF74 ELISA KitRat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) ELISA KitN-鑻勬哀缇板熀-L-璋锋皑閰肝?钁¤悇绯栭啗閰搁叾锛埼?glucuronidase锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

ZRANB2 ELISA KitRat NOS2/iNOS(Nitric Oxide Synthase 2, Inducible) ELISA Kit涔欓叞涓欓叜闀嶇晶閰搁叝閰讹紙carboxylesterase锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

ZNF746 ELISA KitRat INHbA(Inhibin beta A) ELISA Kit涔欓叞涓欓叜闀嶇粏鑳炶胺姘ㄩ叞鍗婅儽姘ㄩ吀鍚堟垚閰讹紙glutamyl systeine synthetase锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

ZRANB3 ELISA KitRat Npy(Pro-neuropeptide Y) ELISA Kit涔欓叞涓欓叜闀嶇粍缁囪胺姘ㄩ叞鍗婅儽姘ㄩ吀鍚堟垚閰讹紙glutamyl systeine synthetase锛夋椿鎬ф瘮鑹叉硶瀹氶噺妫€娴嬭瘯鍓傜洅

缁嗚優鍩瑰吇鐨勪紭鐐癸細

1.鐮旂┒鐨勫璞℃槸娲荤粏鑳濣/span>

鍦ㄥ疄楠岃繃绋嬩腑锛屾牴鎹姹傚彲濮嬬粓淇濇寔缁嗚優娲诲姏锛屽苟鍙暱鏃堕棿鐩戞帶銆佹娴嬬敋鑷冲畾閲忚瘎浼颁竴閮ㄥ垎娲荤粏鑳炵殑鎯呭喌锛屽寘鎷椿缁嗚優鐨勫舰鎬併€佺粨鏋勩€佺敓鍛芥椿鍔ㄧ瓑銆侟/span>

2.鐮旂┒鏉′欢鍙互浜轰负鎺у埗

pH銆佹俯搴︺€佹哀姘斻€佷簩姘у寲纰炽€佸紶鍔涚瓑鐗╃悊鍖栧鐨勬潯浠讹紝鍙互鏍规嵁瀹為檯闇€瑕佽繘琛屼汉涓虹殑鎺у埗锛屽悓鏃讹紝鍙互鏂藉姞鍖栧銆佺墿鐞嗐€佺敓鐗╃瓑鍥犵礌浣滀负鏉′欢鑰岃繘琛屽疄楠岃瀵燂紝杩欎簺鍥犵礌鍚屾牱鍙互澶勪簬涓ユ牸鎺у埗涔嬩笅銆侟/span>

3.鐮旂┒鐨勬牱鏈彲浠ヨ揪鍒版瘮杈冨潎涓€鎬?/span>

閫氳繃缁嗚優鍩瑰吇涓€瀹氫唬鏁板悗锛屾墍寰楀埌鐨勭粏鑳炵郴鍒欏彲浠ヨ揪鍒板潎涓€鎬ц€屽睘浜庡悓涓€绫诲瀷鐨勭粏鑳烇紝闇€瑕佹椂锛屽彲閲囩敤鍏嬮殕鍖栫瓑鏂规硶浣跨粏鑳炶揪鍒扮函鍖栥€侟/span>

4.鐮旂┒鍐呭渚夸簬瑙傚療銆佹娴嬪拰璁板綍

閲囩敤鍚勭鐮旂┒鎶€鏈細濡傚€掔疆鐢熺墿鏄惧井闀溿€佽崸鍏夋樉寰暅銆佺數瀛愭樉寰暅銆佹祦寮忕粏鑳炴湳銆佹縺鍏夊叡鐒︽樉寰暅銆佸厤鐤粍缁囧寲瀛︺€佸師浣嶆潅浜ゃ€佸悓浣嶇礌鏍囪绛夊悇绉嶄华鍣ㄨ澶囥€侟/span>