鐗规€э細

NAMALWA(Burkitt's 娣嬪反鐦ょ粏鑳?瀹夊叏鎬э細鎵€鏈夎偪鐦ゅ拰鐥呰浆鏌撶殑缁嗚優鍧囪涓烘湁娼滃湪鐨勭敓鐗╁嵄瀹虫€э紝蹇呴』鍦ㄤ簩绾х敓鐗╁畨鍏ㄥ彴鍐呮搷浣滐紝骞惰娉ㄦ剰闃叉姢锛屾墍鏈夊簾娑插強鎺ヨЕ杩囨缁嗚優鐨勫櫒鐨块渶楂樺帇鐏弻鍚庢柟鑳戒涪寮冦€傛敹鍒扮粏鑳炲悗锛屽湪鍊掔疆闀滀笅濂芥槸鍦?/span>4X鐗╅暅)瑙傚療鏁翠釜缁嗚優鐢熼暱鎯呭喌

锛堜竴锛夊鏋滅粏鑳炴湭闀挎弧锛岀敤75%閰掔簿鍠锋磼鏁翠釜鐡舵秷鍚庢斁鍒拌秴鑿屽彴鍐咃紝涓ユ牸鏃犺弻鎿嶄綔锛屾墦寮€缁嗚優鍩瑰吇鐡讹紝鍚稿嚭鍩瑰吇娑诧紝鎹 10ml鏂伴矞鍩瑰吇娑插悗缁х画鍩瑰吇銆侟/span>

(浜?濡傛灉缁嗚優宸查暱婊★紝鍗冲彲杩涜浼犱唬鍩瑰吇銆傚叿浣撴楠ゅ涓嬶細

1. 寮冨幓鍩瑰吇娑诧紝鐢≒BS锛堜笉鍚挋,闀佺瀛愶級娲?-2娆°€侟/span>

2. 鍔?ml娑堝寲娑诧紙0.25%Trypsin-0.53mM EDTA锛変簬鍩瑰吇鐡朵腑锛屽€掓斁浜?7鈩冨煿鍏荤1-3鍒嗛挓棰勭儹锛屼箣鍚庣炕杞煿鍏荤摱10-30绉掑悗锛屽湪鍊掔疆鏄惧井闀滀笅瑙傚療缁嗚優娑堝寲鎯呭喌锛岃嫢缁嗚優澶ч儴鍒嗗彉鍦嗭紝杩呴€熸嬁鍥炴搷浣滃彴锛岃交鏁插嚑涓嬪煿鍏荤摱鍚庡姞灏戦噺瀹屽叏鍩瑰吇鍩虹粓姝㈡秷鍖栥€侟/span>

3. 鎸?-8ml/鐡惰ˉ鍔犲畬鍏ㄥ煿鍏诲熀锛岃交杞绘墦鍖€鍚庡惛鍑轰竴鍗婏紝鍒嗗埌鏂扮殑鍩瑰吇鐡朵腑銆侟/span>

浜у搧鍚嶇О	浜у搧绠€绉?/span>	璐у彿
NAMALWA(Burkitt's 娣嬪反鐦ょ粏鑳?	NAMALWA	GOY-X6328

鐗规€э細

瀹夊叏鎬э細鎵€鏈夎偪鐦ゅ拰鐥呰浆鏌撶殑缁嗚優鍧囪涓烘湁娼滃湪鐨勭敓鐗╁嵄瀹虫€э紝蹇呴』鍦ㄤ簩绾х敓鐗╁畨鍏ㄥ彴鍐呮搷浣滐紝骞惰娉ㄦ剰闃叉姢锛屾墍鏈夊簾娑插強鎺ヨЕ杩囨缁嗚優鐨勫櫒鐨块渶楂樺帇鐏弻鍚庢柟鑳戒涪寮冦€傛敹鍒扮粏鑳炲悗锛屽湪鍊掔疆闀滀笅濂芥槸鍦?/span>4X鐗╅暅)瑙傚療鏁翠釜缁嗚優鐢熼暱鎯呭喌

锛堜竴锛夊鏋滅粏鑳炴湭闀挎弧锛岀敤75%閰掔簿鍠锋磼鏁翠釜鐡舵秷鍚庢斁鍒拌秴鑿屽彴鍐咃紝涓ユ牸鏃犺弻鎿嶄綔锛屾墦寮€缁嗚優鍩瑰吇鐡讹紝鍚稿嚭鍩瑰吇娑诧紝鎹 10ml鏂伴矞鍩瑰吇娑插悗缁х画鍩瑰吇銆侟/span>

(浜?濡傛灉缁嗚優宸查暱婊★紝鍗冲彲杩涜浼犱唬鍩瑰吇銆傚叿浣撴楠ゅ涓嬶細

1. 寮冨幓鍩瑰吇娑诧紝鐢≒BS锛堜笉鍚挋,闀佺瀛愶級娲?-2娆°€侟/span>

2. 鍔?ml娑堝寲娑诧紙0.25%Trypsin-0.53mM EDTA锛変簬鍩瑰吇鐡朵腑锛屽€掓斁浜?7鈩冨煿鍏荤1-3鍒嗛挓棰勭儹锛屼箣鍚庣炕杞煿鍏荤摱10-30绉掑悗锛屽湪鍊掔疆鏄惧井闀滀笅瑙傚療缁嗚優娑堝寲鎯呭喌锛岃嫢缁嗚優澶ч儴鍒嗗彉鍦嗭紝杩呴€熸嬁鍥炴搷浣滃彴锛岃交鏁插嚑涓嬪煿鍏荤摱鍚庡姞灏戦噺瀹屽叏鍩瑰吇鍩虹粓姝㈡秷鍖栥€侟/span>

3. 鎸?-8ml/鐡惰ˉ鍔犲畬鍏ㄥ煿鍏诲熀锛岃交杞绘墦鍖€鍚庡惛鍑轰竴鍗婏紝鍒嗗埌鏂扮殑鍩瑰吇鐡朵腑銆侟/span>

缁嗚優鐗规€э細

1)缁勭粐鏉ユ簮浜庡疄楠屽姩鐗╃殑姝ｅ父鐪肩粍缁囥€侟/span>

2)缁嗚優閴村畾锛氱粏鑳炶铔嬬櫧-18(CK-18)鍏嶇柅鑽у厜鏌撹壊涓洪槼鎬с€侟/span>

3)缁忛壌瀹氱粏鑳炵函搴﹂珮浜?0%銆侟/span>

4)涓嶅惈鏈 HIV-1銆 HBV銆丠CV銆佹敮鍘熶綋銆佺粏鑿屻€侀叺姣嶅拰鐪熻弻銆侟/span>

5)缁嗚優鐢熼暱鏂瑰紡锛氫笂鐨牱锛屼笉瑙勫垯缁嗚優锛岃创澹佸煿鍏汇€侟/span>

娉ㄦ剰浜嬮」锛欬/span>

1銆佽瀵熺粏鑳瀦ui濂藉湪浣庡€嶉暅锛?鎴?X鐗╅暅)涓嬭繘琛岋紝鍚﹀垯涓嶈兘鍑嗙‘鍒ゆ柇缁嗚優鐨勪紶浠ｅ瘑搴︺€傜湅缁嗚優鐨勫舰鎬佽鍦?0X鎴?0X鐗╅暅涓嬨€侟/span>

2銆佺摱涓繍杈撳煿鍏诲熀涓嶈兘閲嶅鍐嶇敤,璇锋崲鐢ㄥ姞鍙屾姉鐨勬柊鍩瑰吇鍩猴紝缁嗚優鍐诲瓨鍚?鍩瑰吇鍩轰腑鍙笉鍔犱换浣曟姉鐢熺礌銆侟/span>

3銆佹湁浜涚粏鑳炶创澹佷笉鐗紝濡傚彂鐜拌创澹佺粏鑳炴湁鑴辫惤锛屽彲绂诲績鍚规墦鍚庢帴绉嶅埌鏂扮摱鍐呫€侟/span>

4銆佹敹鍒扮粏鑳炲悗锛岃嫢鍙戠幇鍩瑰吇鐡剁牬鎹熴€佹湁娑叉孩鍑哄強缁嗚優鏈夋薄鏌擄紝璇峰強鏃朵笌鎴戜滑銆侟/span>

GFOD1 (Glucose-fructose oxidoreductase domain-containing protein 1) ELISA KITMouse HD3(Histone Deacetylase 3) ELISA KitN,O-鍙?涓夌敳鍩虹鐑峰熀)涔欓叞鑳哄喕鍒囩墖鐗规仼甯冨皵锛圱URNBULL锛夐搧锛堜簩浠凤級鏌撹壊璇曞墏鐩扅/span>

MRGPRX2 (Mas-related G-protein coupled receptor member X2) ELISA KITMouse PDIA4(Protein disulfide-isomerase A4) ELISA KitN,O-鍙?涓夌敳鍩虹鐑峰熀)涔欓叞鑳虹粏鑳炵壒鎭╁竷灏旓紙TURNBULL锛夐搧锛堜簩浠凤級鏌撹壊璇曞墏鐩扅/span>

LGALSL (Galectin-related protein) ELISA KITMouse 尾-actin(Beta actin) ELISA KitN-涔欓叞-D-鍗婁钩绯栬兒鐭宠湣鍒囩墖鐨皵鏂紙PERLS-DAB锛夐潪琛€绾㈢礌閾侊紙涓変环锛夊己鍖栨煋鑹茶瘯鍓傜洅

GLCE (D-glucuronyl C5-epimerase) ELISA KITMouse CEBPD(CCAAT/enhancer Binding Protein(C/EBP), delta) ELISA KitN-涔欓叞-D-鍗婁钩绯栬兒鍐诲垏鐗囩毊灏旀柉锛圥ERLS-DAB锛夐潪琛€绾㈢礌閾侊紙涓変环锛夊己鍖栨煋鑹茶瘯鍓傜洅

GLMN (Glomulin) ELISA KITMouse CSF1R(Macrophage colony-stimulating factor 1 receptor) ELISA KitN,N-浜屼箼鍩哄鑻簩鑳虹粏鑳炵毊灏旀柉锛圥ERLS-DAB锛夐潪琛€绾㈢礌閾侊紙涓変环锛夊己鍖栨煋鑹茶瘯鍓傜洅

GPR65 (Psychosine receptor) ELISA KITMouse ICTP(Cross-linked C-terminal Telopeptide of Type I Collagen) ELISA KitN,N-浜屼箼鍩哄鑻簩鑳虹煶铚″垏鐗囩壒鎭╁竷灏旓紙TURNBULL-DAB锛夐潪琛€绾㈢礌閾侊紙浜屼环锛夊己鍖栨煋鑹茶瘯鍓傜洅

ARHGAP35 (Rho GTPase-activating protein 35) ELISA KITMouse ADAM10(Disintegrin and metalloproteinase domain-containing protein 10) ELISA Kit钀橀厷AS-D鍐诲垏鐗囩壒鎭╁竷灏旓紙TURNBULL-DAB锛夐潪琛€绾㈢礌閾侊紙浜屼环锛夊己鍖栨煋鑹茶瘯鍓傜洅

GFOD1 (Glucose-fructose oxidoreductase domain-containing protein 1) ELISA KITMouse CD26(Dipeptidyl peptidase 4) ELISA Kit钀橀厷AS-D缁嗚優鐗规仼甯冨皵锛圱URNBULL-DAB锛夐潪琛€绾㈢礌閾侊紙浜屼环锛夊己鍖栨煋鑹茶瘯鍓傜洅

PPPDE1 ELISA KitTestis 鑲跨槫鎶戝埗鍩哄洜Testin鎶椾綋姘熷寲閿?II)Korser姘忚倝姹｜/span>

PPP6C ELISA KitZNF288/ZBTB20 閿屾寚铔嬬櫧288鎶椾綋鍥涗箼鍩轰箼浜岄唶鍗曠敳閰疢R-VP

PPP4C ELISA KitALOX15/15 Lipoxygenase 1 鑺辩敓鍥涚儻閰?5鑴傛哀鍚堥叾1鎶椾綋鍥涗箼鍩轰箼浜岄唶鍗曠敳閰泲鐧借儴姘达紙鑹叉皑閰歌倝姹わ級

PPP5C ELISA KitABI3BP ABI鍩哄洜瀹舵棌鎴愬憳3缁撳悎铔嬬櫧鎶椾綋鍙嶅紡-2-宸辩儻閱涘崼鐭涢唶鍗婂浐浣掽/span>

PPP1R3F ELISA KitMTCBP1/ADI1 鑶滃瀷鍩鸿川閲戝睘铔嬬櫧閰惰優璐ㄥ熬缁撳悎铔嬬櫧1鎶椾綋鍙嶅紡-2-宸辩儻閱涘北姊ㄩ唶鍙戦叺绠狘/span>

PPT1 ELISA KitBCMP11/AGR3 涔宠吅鐧岃啘铔嬬櫧11鎶椾綋锛堝墠姊害鍚屾簮铔嬬櫧2锛夌壒缇庢眬锛堟柊鍨嬮潻鍏版皬闃存€ц弻鎶楃敓绱狅級涓欎簩閰哥洂

PPP1R7 ELISA KitALDH1A1 鑳炶川涔欓啗鑴辨阿閰?鎶椾綋姘у寲閾?III,IV)姘存潹鑻峰彂閰电

PPP3CB ELISA KitABH8/ALKBH8 AlkB鍚屾簮铔嬬櫧8鎶椾綋姘у寲閾?III,IV)璧栨皑閰歌劚缇ч叾鑲夋堡

瀹為獙鎶ュ憡锛欬/span>

涓€銆佸垎绂讳笌鍩瑰吇锛欬/span>

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2銆佸線缁勭粐鍧椾腑鍔犲叆4 mL閰舵秷鍖栨恫锛?.1% 鑳伴叾鍜?.1% I鍨嬭兌鍘熼叾锛夛紝娣锋偓10s锛岀疆37鈩冩潯浠朵笅娑堝寲10min锛屼箣鍚庣敤婊寸鍚规墦鍒舵垚鍗曠粏鑳炴偓娑诧紝鑷劧娌夋穩骞舵敹闆嗕笂娓咃紝鐢ㄥ惈10% FBS鍩瑰吇鍩虹粓姝㈡秷鍖栧悗4鈩冩斁缃紱

3銆佸墿涓嬬殑缁勭粐鍐嶅姞鍏?锝?mL閰舵秷鍖栨恫锛屾贩鎮?0s锛岀疆37鈩冩秷鍖?0 min鍚庯紝鎸変笂杩版柟娉曟敹闆嗕笂娓呭苟缁堟娑堝寲鍚?鈩冩斁缃紝閲嶅姝ゆ楠?-3娆★紝鐩磋嚦缁勭粐瀹屽叏琚秷鍖栵紱

4銆佺敤200鐩笉閿堥挗绛涚綉杩囨护缁嗚優娑堝寲娑诧紝1200r/min 绂诲績10min锛屽純鍘讳笂娓咃紝娌夋穩缁嗚優鐢ㄥ惈鏈?0锛 FBS DMEM/F12鍩瑰吇鍩烘贩鎮紝鎺ョ浜?5cm2鍩瑰吇鐡讹紝鏀剧疆浜?7鈩 锛?锛匔O2 鍩瑰吇绠变腑鍩瑰吇锛汓/span>

5銆佸樊閫熻创澹?h鍚庯紝鍚稿嚭鍩瑰吇鍩猴紝鎸夊疄楠岄渶瑕佹帴绉嶄簬6瀛旀澘涓户缁煿鍏伙紱

浜屻€佸厤鐤崸鍏夐壌瀹氾細

1銆佸緟蹇冩埧鑲岀粏鑳炵敓闀胯嚦80%铻嶅悎鏃讹紝寮冨幓鍩瑰吇鍩猴紝鐢ㄦ俯鑲茬殑PBS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庣敤4%澶氳仛鐢查啗鍦ㄥ娓╂潯浠朵笅鍥哄畾缁嗚優15min锛汓/span>

2銆丳BS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庡湪4鈩冩潯浠朵笅锛岀敤0.1锛匱riton X-100閫忚啘15min锛汓/span>

3銆丳BS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庡湪瀹ゆ俯鏉′欢涓嬶紝鐢?% BSA灏侀棴缁嗚優30min锛汓/span>

4銆佹寜1: 100鐨勬瘮渚嬬█閲娢?actin涓€鎶楋紙Abbioscience鍏徃锛夛紝鐒跺悗灏嗗叾鏀惧湪4鈩冪涓鑲茬粏鑳炶繃澶滐紱

5銆丳BS鍐叉礂缁嗚優3娆★紝姣忔10min锛屾寜1锛?50鐨勬瘮渚嬬█閲婃姉伪-actin鐨勪簩鎶楋紝 37鈩冩潯浠朵笅鏀剧疆1h锛汓/span>

6銆佺敤PBS鍐叉礂3娆★紝姣忔10min锛屾渶鍚庡湪鍊掔疆鑽у厜鏄惧井闀滀笅瑙傚療鍥惧儚骞舵媿鐓с€侟/span>