鎷瓙鐥呮瘨杩愯緭娑秉/span>B锛圴iral Transport Medium B锛孷TM-B锛堻/span>PCR鍙嶅簲鐨勭壒寮傛€у喅瀹氬洜绱犱负锛欬/span>

銆€銆€銆€鈶犲紩鐗╀笌妯℃澘DNA鐗瑰紓姝ｇ‘鐨勭粨鍚堬紱

銆€銆€銆€鈶＄⒈鍩洪厤瀵瑰師鍒欙紱

銆€銆€銆€鈶aq DNA鑱氬悎閰跺悎鎴愬弽搴旂殑蹇犲疄鎬э紱

銆€銆€銆€鈶ｉ澏鍩哄洜鐨勭壒寮傛€т笌淇濆畧鎬с€侟/span>

鍏朵腑寮曠墿涓庢ā鏉跨殑姝ｇ‘缁撳悎鏄叧閿€傚紩鐗╀笌妯℃澘鐨勭粨鍚堝強寮曠墿閾剧殑寤朵几鏄伒寰⒈鍩洪厤瀵瑰師鍒欑殑銆傝仛鍚堥叾鍚堟垚鍙嶅簲鐨勫繝瀹炴€у強Taq DNA鑱氬悎閰惰€愰珮娓╂€э紝浣垮弽搴斾腑妯℃澘涓庡紩鐗╃殑缁撳悎(澶嶆€?鍙互鍦ㄨ緝楂樼殑娓╁害涓嬭繘琛岋紝缁撳悎鐨勭壒寮傛€уぇ澶у鍔狅紝琚墿澧炵殑闈跺熀鍥犵墖娈典篃灏辫兘淇濇寔寰堥珮鐨勬纭害銆傚啀閫氳繃閫夋嫨鐗瑰紓鎬у拰淇濆畧鎬ч珮鐨勯澏鍩哄洜鍖猴紝鍏剁壒寮傛€х▼搴﹀氨鏇撮珮銆侟/span>

(2) 鐏垫晱搴﹂珮

PCR浜х墿鐨勭敓鎴愰噺鏄互鎸囨暟鏂瑰紡澧炲姞鐨勶紝鑳藉皢鐨厠(pg=10-12g)閲忕骇鐨勮捣濮嬪緟娴嬫ā鏉挎墿澧炲埌寰厠(ug=10-6g)姘村钩銆傝兘浠?00涓囦釜缁嗚優涓鍑轰竴涓澏缁嗚優锛涘湪鐥呮瘨鐨勬娴嬩腑锛孭CR鐨勭伒鏁忓害鍙揪3涓猂FU(绌烘枒褰㈡垚鍗曚綅)锛涘湪缁嗚弻瀛︿腑zui灏忔鍑虹巼涓?涓粏鑿屻€侟/span>

(3) 绠€渚裤€佸揩閫烖/span>

PCR鍙嶅簲鐢ㄨ€愰珮娓╃殑Taq DNA鑱氬悎閰讹紝涓€娆℃€у湴灏嗗弽搴旀恫鍔犲ソ鍚庯紝鍗冲湪DNA鎵╁娑插拰姘存荡閿呬笂杩涜鍙樻€?閫€鐏?寤朵几鍙嶅簲锛屼竴鑸湪2锝? 灏忔椂瀹屾垚鎵╁鍙嶅簲銆傛墿澧炰骇鐗╀竴鑸敤鐢垫吵鍒嗘瀽锛屼笉涓€瀹氳鐢ㄥ悓浣嶇礌锛屾棤鏀惧皠鎬ф薄鏌撱€佹槗鎺ㄥ箍銆侟/span>

(4) 瀵规爣鏈殑绾害瑕佹眰浣嶞/span>

涓嶉渶瑕佸垎绂荤梾姣掓垨缁嗚弻鍙婂煿鍏荤粏鑳烇紝DNA 绮楀埗鍝佸強鎬籖NA鍧囧彲浣滀负鎵╁妯℃澘銆傚彲鐩存帴鐢ㄤ复搴婃爣鏈琛€娑层€佷綋鑵旀恫銆佹礂鍡芥恫銆佹瘺鍙戙€佺粏鑳炪€佹椿PCR鎶€鏈璁恒€侟/span>

銆愬妫€楠岀粨鏋滅殑瑙ｉ噴銆慄/span>

1. 瀹為獙瀹ょ幆澧冩薄鏌撱€佽瘯鍓傛薄鏌撱€佹牱鍝佷氦鍙夋薄鏌撲細鍑虹幇鍋囬槼鎬х粨鏋滐紱

2. 璇曞墏杩愯緭銆佷繚瀛樹笉褰撴垨璇曞墏閰嶅埗涓嶅噯纭紩璧风殑璇曞墏妫€娴嬫晥鑳戒笅闄嶏紝鍙兘浼氬鑷村嚭鐜板亣闃存€х粨鏋溿€侟/span>

浜у搧鍚嶇О	鑻辨枃鍚嶇О	缂栧彿
鎷瓙鐥呮瘨杩愯緭娑睟锛圴iral Transport Medium B锛孷TM-B锛堻/span>	璇﹁璇存槑涔?/span>

瑙勬牸锛欬/span>50T
鍒嗙被锛欬/span>PCR妫€娴嬭瘯鍓傜洅

鍌ㄥ瓨鏉′欢锛欬/span>-20鈩冮伩鍏変繚瀛橈紝閬垮厤鍙嶅鍐昏瀺銆侟/span>

杩愯緭锛氫綆娓┿€侀伩鍏夈€侟/span>

鏈夋晥鏈燂細涓€骞滁/span>

璐ф湡锛氱幇璐?/span>PCR璇曞墏鐩掔殑鏈夋晥鏈熶竴鑸负1骞达紝杩欐槸鎸囧湪閫傚綋鐨勫偍瀛樻俯搴︿笅銆傛牳閰告墿澧為儴鍒嗙殑璇曞墏涓€鑸璐瓨浜?20鈩冧笅锛屼骇鐗╂娴嬮儴鍒嗚瘯鍓傚垯璐瓨浜?-8鈩冦€傚敖閲忛伩鍏嶅弽澶嶅喕铻嶃€侟/span>

銆愮粨鏋滃垎鏋愭潯浠惰瀹氥€慄/span>

u 鍩虹嚎锛圔aseline锛夎瀹氾細搴旈€夋嫨璇ユ瀹為獙鎸囨暟鎵╁鍓嶆墍鏈夋牱鏈崸鍏変俊鍙锋瘮杈冪ǔ瀹氱殑涓€娈靛尯鍩燂紙鎵€鏈夋牱鏈崸鍏変俊鍙锋棤杈冨ぇ娉㈠姩锛夛紝璧峰鐐癸紙Start锛夊簲閬垮紑鑽у厜閲囬泦璧峰闃舵鐨勪俊鍙锋尝鍔紝缁堟鐐癸紙End锛夊簲姣旀渶鏃╁嚭鐜版寚鏁版墿澧炵殑鏍锋湰Ct鍊煎噺灏?~3涓惊鐜紝寤鸿鍩虹嚎璁句负6~15銆侟/span>

u 闃堝€?Threshold)璁惧畾锛氬皢闃堝€肩嚎璁惧畾鍦ㄥ垰濂借秴杩囨甯搁槾鎬ц川鎺у搧鎵╁鏇茬嚎鐨勬渶楂樼偣銆侟/span>
銆愯川鎺ф爣鍑嗐€慄/span>

1. 闃存€ц川鎺у搧鐨勬娴嬬粨鏋滃簲涓洪槾鎬э紝鏃犳寚鏁版墿澧炴湡锛孋t=40鎴栨棤Ct锛汓/span>

2. 闃虫€ц川鎺у搧鐨勬娴嬬粨鏋滃簲涓洪槼鎬э紝鏈夋槑鏄剧殑鎸囨暟鎵╁鏈燂紝Ct鍊煎簲灏忎簬绛変簬35锛汓/span>

3. 浠ヤ笂瑕佹眰闇€鍦ㄤ竴娆″疄楠屼腑鍚屾椂婊¤冻锛屽惁鍒欒娆″疄楠岃涓烘棤鏁堛€侟/span>

銆愮粨鏋滃垽鏂€慄/span>

1. 闃虫€э細鏈夋槑鏄剧殑鎸囨暟鎵╁鏈燂紝涓擟t锛?8锛汓/span>

2.鍙枒锛氭湁鏄庢樉鐨勬寚鏁版墿澧炴湡锛屼笖38鈮t<40锛屾鏃舵牱鏈负鍙枒鏍锋湰锛涘浜庡彲鐤戞牱鏈缓璁鏍镐竴娆★紝鑻ュ鏍哥粨鏋淐t<40涓旀湁鎸囨暟鎵╁鏈燂紝鍒欏垽瀹氫负闃虫€э紝鍚﹀垯鍒ゅ畾涓洪槾鎬э紱

3. 闃存€э細鏃犳寚鏁版墿澧炴湡锛孋t=40鎴栨棤Ct鍊煎潎鍒ゅ畾涓洪槾鎬ф牱鏈€侟/span>

MLX 杞寲鍥犲瓙鏍疯泲鐧?鎶椾綋鐜鑿岀礌D

MLC3F/MYL1/3 鑲岀悆铔嬬櫧杞婚摼1/3鎶椾綋鐜鑿岀礌H

Phospho-MYL(Ser19) 纾烽吀鍖栬倢鐞冭泲鐧借交閾?鎶椾綋鐜粍鑺唶

MYL3/Myosin light chain 3 鑲岀悆铔嬬櫧杞婚摼3鎶椾綋鐜哀闀挎槬纰包/span>

MAX protein Myc鍩哄洜鐩稿叧X鍥犲瓙鎶椾綋榛勬煆纰包/span>

phospho-MAX protein(Tyr123) 纾烽吀鍖朚yc鍩哄洜鐩稿叧X鍥犲瓙鎶椾綋榛勮眴榛勮嫹

Myt1 楂撻灅杞綍鍥犲瓙1鎶椾綋榛勮眴榛勭礌

Phospho-Myt1(Ser83) 纾烽吀鍖栭珦闉樿浆褰曞洜瀛?鎶椾綋榛勭嫭绱燘

MYL6 鑲岀悆铔嬬櫧杞婚摼6鎶椾綋榛勫崕纰包/span>

phospho-MYL6(Tyr29+Ser30) 纾烽吀鍖栬倢鐞冭泲鐧借交閾?鎶椾綋榛勮姫鐨傝嫹 I
鎷瓙鐥呮瘨杩愯緭娑秉/span>B锛圴iral Transport Medium B锛孷TM-B锛堻/span>Ntn1 杞寸獊瀵煎悜鍥犲瓙1鎶椾綋姘㈡捍閰告姒旂⒈

Nur77/GFRP 瀛ゅ効鏍稿彈浣撹泲鐧絅ur77鎶椾綋琛ラ鑴傚畾

Phospho-Nur77(Ser351) 纾烽吀鍖栧鍎挎牳鍙椾綋铔嬬櫧Nur77鎶椾綋琛ラ鑴傞厷

NuMA 鏍告湁涓濆垎瑁傚櫒NuMA铔嬬櫧鎶椾綋琛ラ鑴傜敳绱燑/span>

Phospho-NuMA (Ser395) 纾烽吀鍖栨牳鏈変笣鍒嗚鍣∟uMA铔嬬櫧鎶椾綋琛ラ鑴傜礌

NUMB 鑶滅浉鍏宠泲鐧絅umb鎶椾綋琛ラ鑴備箼绱燑/span>

Phospho-Numb (Ser276) 纾烽吀鍖栬啘鐩稿叧铔嬬櫧Numb鎶椾綋鑻嶆湳鑻稟

NUP54 NUP54鎶椾綋鑻嶆湳绱燑/span>

Nurr1 鏍稿彈浣撶浉鍏冲洜瀛?1鎶椾綋鑽変箤鐢茬礌

OAS1 瀵¤吅鑻烽吀鍚堟垚閰?1宀戦叜
缁勬垚鍙婅瘯鍓傞厤鍒迭/span>:

1銆侀叾鏍囨澘锛氫竴鍧楋紙96瀛旓級

2銆 鏍囧噯鍝侊紙鍐诲共鍝侊級锛 2鐡讹紝璇蜂复鐢ㄥ墠15鍒嗛挓鍐呴厤鍒躲€傛瘡鐡朵互鏍峰搧绋€閲婃恫绋€閲婅嚦0.5ml锛岀洊濂藉悗瀹ゆ俯闈欑疆澶х害10鍒嗛挓锛屽悓鏃跺弽澶嶉鍊?鎼撳姩浠ュ姪婧惰В锛屽叾娴撳害涓?00 U/L锛岀劧鍚庡仛绯诲垪鍊嶆瘮绋€閲婏紙娉細涓嶈鐩存帴鍦ㄦ澘涓繘琛屽€嶆瘮绋€閲婏級锛屽垎鍒厤鍒舵垚200 U/L锛?00 U/L锛?0 U/L锛?5 U/L锛?2.5 U/L锛?.25 U/L锛?.12 U/L锛屾牱鍝佺█閲婃恫鐩存帴浣滀负绌虹櫧瀛 0 U/L銆傚閰嶅埗100 U/L鏍囧噯鍝侊細鍙?.3ml 锛堜笉瑕佸皯浜?.3ml 锛?00 U/L鐨勪笂杩版爣鍑嗗搧鍔犲叆鍚湁0.3ml鏍峰搧绋€閲婃恫鐨凟ppendorf绠′腑锛屾贩鍖€鍗冲彲锛屽叾浣欐祿搴︿互姝ょ被鎺ㄣ€侟/span>

3銆 鏍峰搧绋€閲婃恫锛?脳20ml銆侟/span>

4銆 妫€娴嬬█閲婃恫A锛?脳10ml銆侟/span>

5銆 妫€娴嬬█閲婃恫B锛?脳10ml銆侟/span>