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2)缁嗚優閴村畾锛氱粏鑳炶铔嬬櫧-18(CK-18)鍏嶇柅鑽у厜鏌撹壊涓洪槼鎬с€侟/span>

3)缁忛壌瀹氱粏鑳炵函搴﹂珮浜?0%銆侟/span>

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浜у搧鍚嶇О	浜у搧绠€绉?/span>	璐у彿
C666-1-RFP鑽у厜鏍囪缁嗚優鏍?/span>	C666-1-RFP	GOY-N0051

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锛堜竴锛夊鏋滅粏鑳炴湭闀挎弧锛岀敤75%閰掔簿鍠锋磼鏁翠釜鐡舵秷姣掑悗鏀惧埌瓒呰弻鍙板唴锛屼弗鏍兼棤鑿屾搷浣滐紝鎵撳紑缁嗚優鍩瑰吇鐡讹紝鍚稿嚭鍩瑰吇娑诧紝鎹 10ml鏂伴矞鍩瑰吇娑插悗缁х画鍩瑰吇銆侟/span>

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3. 鎸?-8ml/鐡惰ˉ鍔犲畬鍏ㄥ煿鍏诲熀锛岃交杞绘墦鍖€鍚庡惛鍑轰竴鍗婏紝鍒嗗埌鏂扮殑鍩瑰吇鐡朵腑銆侟/span>

娉ㄦ剰浜嬮」锛欬/span>

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3銆佹湁浜涚粏鑳炶创澹佷笉鐗紝濡傚彂鐜拌创澹佺粏鑳炴湁鑴辫惤锛屽彲绂诲績鍚规墦鍚庢帴绉嶅埌鏂扮摱鍐呫€侟/span>

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瀹為獙鎶ュ憡锛欬/span>

涓€銆佸垎绂讳笌鍩瑰吇锛欬/span>

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2銆佸線缁勭粐鍧椾腑鍔犲叆4 mL閰舵秷鍖栨恫锛?.1% 鑳伴叾鍜?.1% I鍨嬭兌鍘熼叾锛夛紝娣锋偓10s锛岀疆37鈩冩潯浠朵笅娑堝寲10min锛屼箣鍚庣敤婊寸鍚规墦鍒舵垚鍗曠粏鑳炴偓娑诧紝鑷劧娌夋穩骞舵敹闆嗕笂娓咃紝鐢ㄥ惈10% FBS鍩瑰吇鍩虹粓姝㈡秷鍖栧悗4鈩冩斁缃紱

3銆佸墿涓嬬殑缁勭粐鍐嶅姞鍏?锝?mL閰舵秷鍖栨恫锛屾贩鎮?0s锛岀疆37鈩冩秷鍖?0 min鍚庯紝鎸変笂杩版柟娉曟敹闆嗕笂娓呭苟缁堟娑堝寲鍚?鈩冩斁缃紝閲嶅姝ゆ楠?-3娆★紝鐩磋嚦缁勭粐瀹屽叏琚秷鍖栵紱

4銆佺敤200鐩笉閿堥挗绛涚綉杩囨护缁嗚優娑堝寲娑诧紝1200r/min 绂诲績10min锛屽純鍘讳笂娓咃紝娌夋穩缁嗚優鐢ㄥ惈鏈?0锛 FBS DMEM/F12鍩瑰吇鍩烘贩鎮紝鎺ョ浜?5cm2鍩瑰吇鐡讹紝鏀剧疆浜?7鈩 锛?锛匔O2 鍩瑰吇绠变腑鍩瑰吇锛汓/span>

5銆佸樊閫熻创澹?h鍚庯紝鍚稿嚭鍩瑰吇鍩猴紝鎸夊疄楠岄渶瑕佹帴绉嶄簬6瀛旀澘涓户缁煿鍏伙紱

浜屻€佸厤鐤崸鍏夐壌瀹氾細

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2銆丳BS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庡湪4鈩冩潯浠朵笅锛岀敤0.1锛匱riton X-100閫忚啘15min锛汓/span>

3銆丳BS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庡湪瀹ゆ俯鏉′欢涓嬶紝鐢?% BSA灏侀棴缁嗚優30min锛汓/span>

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5銆丳BS鍐叉礂缁嗚優3娆★紝姣忔10min锛屾寜1锛?50鐨勬瘮渚嬬█閲婃姉伪-actin鐨勪簩鎶楋紝 37鈩冩潯浠朵笅鏀剧疆1h锛汓/span>

6銆佺敤PBS鍐叉礂3娆★紝姣忔10min锛屾渶鍚庡湪鍊掔疆鑽у厜鏄惧井闀滀笅瑙傚療鍥惧儚骞舵媿鐓с€侟/span>

Chloro[(S)-(-)-2,2'-bis(di-p-tolylphosphino)-1,1'-binaphthyl](p-cymene)ruthenium(II) chloride,[RuCl(p-cymene)((S)-tolbinap)]Cl

(S)-(-)-1-BOC-2-鍚″挴鐑风敳鑵圏/span>

(S)-(-)-1-BOC-2-鍚″挴鐑风敳鑵圏/span>

(R)-(+)-1-Boc-2-鍚″挴鐑风敳鑵圏/span>

(R)-(+)-1-Boc-2-鍚″挴鐑风敳鑵圏/span>

鐮峰寲閾滭/span>

2-(涓夋盁鐢插熀)鑻熀寮傛鞍閰?/span>

2-(涓夋盁鐢插熀)鑻熀寮傛鞍閰?/span>

7-涔欏熀鍚插摎

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C666-1-RFP鑽у厜鏍囪缁嗚優鏍?/span>鐢樻皑閰拌倢姘?/span>HPLC鈮?8%100mLsecretory leukocyte protease inhibitor,SLPI ELISA Kit

1,2-鐜哀杈涚兎HPLC鈮?8%100mLsecretory immunoglobulin A,SIgA ELISA Kit

1,2-鐜哀杈涚兎HPLC鈮?8%100mLsecreted phospholipase A2,sPLA2 ELISA Kit

1,2-鐜哀杈涚兎HPLC鈮?8%100mLSFRP5 ELISA Kit

鍗￠┈瑗垮钩HPLC鈮?8%100mLSFRP4 ELISA Kit

鍗￠┈瑗垮钩HPLC鈮?8%100mLSFRP2 ELISA Kit

鐩愯タ骞颤/span>HPLC鈮?8%100mLSFRP1 ELISA Kit