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3)缁忛壌瀹氱粏鑳炵函搴﹂珮浜?0%銆侟/p>

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5)缁嗚優鐢熼暱鏂瑰紡锛氫笂鐨牱锛屼笉瑙勫垯缁嗚優锛岃创澹佸煿鍏汇€侟/p>

浜у搧鍚嶇О	浜у搧绠€绉?/td>	璐у彿
BIU87/ADR浜鸿唨鑳辩檶闃块湁绱犺€愯嵂鏍?/span>	BIU87/ADR	GOY-N0001

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锛堜竴锛夊鏋滅粏鑳炴湭闀挎弧锛岀敤75%閰掔簿鍠锋磼鏁翠釜鐡舵秷姣掑悗鏀惧埌瓒呰弻鍙板唴锛屼弗鏍兼棤鑿屾搷浣滐紝鎵撳紑缁嗚優鍩瑰吇鐡讹紝鍚稿嚭鍩瑰吇娑诧紝鎹 10ml鏂伴矞鍩瑰吇娑插悗缁х画鍩瑰吇銆侟/p>

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2. 鍔?ml娑堝寲娑诧紙0.25%Trypsin-0.53mM EDTA锛変簬鍩瑰吇鐡朵腑锛屽€掓斁浜?7鈩冨煿鍏荤1-3鍒嗛挓棰勭儹锛屼箣鍚庣炕杞煿鍏荤摱10-30绉掑悗锛屽湪鍊掔疆鏄惧井闀滀笅瑙傚療缁嗚優娑堝寲鎯呭喌锛岃嫢缁嗚優澶ч儴鍒嗗彉鍦嗭紝杩呴€熸嬁鍥炴搷浣滃彴锛岃交鏁插嚑涓嬪煿鍏荤摱鍚庡姞灏戦噺瀹屽叏鍩瑰吇鍩虹粓姝㈡秷鍖栥€侟/p>

3. 鎸?-8ml/鐡惰ˉ鍔犲畬鍏ㄥ煿鍏诲熀锛岃交杞绘墦鍖€鍚庡惛鍑轰竴鍗婏紝鍒嗗埌鏂扮殑鍩瑰吇鐡朵腑銆侟/p>

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2銆佺摱涓繍杈撳煿鍏诲熀涓嶈兘閲嶅鍐嶇敤,璇锋崲鐢ㄥ姞鍙屾姉鐨勬柊鍩瑰吇鍩猴紝缁嗚優鍐诲瓨鍚?鍩瑰吇鍩轰腑鍙笉鍔犱换浣曟姉鐢熺礌銆侟/p>

3銆佹湁浜涚粏鑳炶创澹佷笉鐗紝濡傚彂鐜拌创澹佺粏鑳炴湁鑴辫惤锛屽彲绂诲績鍚规墦鍚庢帴绉嶅埌鏂扮摱鍐呫€侟/p>

4銆佹敹鍒扮粏鑳炲悗锛岃嫢鍙戠幇鍩瑰吇鐡剁牬鎹熴€佹湁娑叉孩鍑哄強缁嗚優鏈夋薄鏌擄紝璇峰強鏃朵笌鎴戜滑銆侟/p>

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2銆佸線缁勭粐鍧椾腑鍔犲叆4 mL閰舵秷鍖栨恫锛?.1% 鑳伴叾鍜?.1% I鍨嬭兌鍘熼叾锛夛紝娣锋偓10s锛岀疆37鈩冩潯浠朵笅娑堝寲10min锛屼箣鍚庣敤婊寸鍚规墦鍒舵垚鍗曠粏鑳炴偓娑诧紝鑷劧娌夋穩骞舵敹闆嗕笂娓咃紝鐢ㄥ惈10% FBS鍩瑰吇鍩虹粓姝㈡秷鍖栧悗4鈩冩斁缃紱

3銆佸墿涓嬬殑缁勭粐鍐嶅姞鍏?锝?mL閰舵秷鍖栨恫锛屾贩鎮?0s锛岀疆37鈩冩秷鍖?0 min鍚庯紝鎸変笂杩版柟娉曟敹闆嗕笂娓呭苟缁堟娑堝寲鍚?鈩冩斁缃紝閲嶅姝ゆ楠?-3娆★紝鐩磋嚦缁勭粐瀹屽叏琚秷鍖栵紱

4銆佺敤200鐩笉閿堥挗绛涚綉杩囨护缁嗚優娑堝寲娑诧紝1200r/min 绂诲績10min锛屽純鍘讳笂娓咃紝娌夋穩缁嗚優鐢ㄥ惈鏈?0锛 FBS DMEM/F12鍩瑰吇鍩烘贩鎮紝鎺ョ浜?5cm2鍩瑰吇鐡讹紝鏀剧疆浜?7鈩 锛?锛匔O2 鍩瑰吇绠变腑鍩瑰吇锛汓/p>

5銆佸樊閫熻创澹?h鍚庯紝鍚稿嚭鍩瑰吇鍩猴紝鎸夊疄楠岄渶瑕佹帴绉嶄簬6瀛旀澘涓户缁煿鍏伙紱

浜屻€佸厤鐤崸鍏夐壌瀹氾細

1銆佸緟蹇冩埧鑲岀粏鑳炵敓闀胯嚦80%铻嶅悎鏃讹紝寮冨幓鍩瑰吇鍩猴紝鐢ㄦ俯鑲茬殑PBS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庣敤4%澶氳仛鐢查啗鍦ㄥ娓╂潯浠朵笅鍥哄畾缁嗚優15min锛汓/p>

2銆丳BS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庡湪4鈩冩潯浠朵笅锛岀敤0.1锛匱riton X-100閫忚啘15min锛汓/p>

3銆丳BS鍐叉礂缁嗚優2娆★紝姣忔10min锛岀劧鍚庡湪瀹ゆ俯鏉′欢涓嬶紝鐢?% BSA灏侀棴缁嗚優30min锛汓/p>

4銆佹寜1: 100鐨勬瘮渚嬬█閲娢?actin涓€鎶楋紙Abbioscience鍏徃锛夛紝鐒跺悗灏嗗叾鏀惧湪4鈩冨啺绠变腑瀛佃偛缁嗚優杩囧锛汓/p>

5銆丳BS鍐叉礂缁嗚優3娆★紝姣忔10min锛屾寜1锛?50鐨勬瘮渚嬬█閲婃姉伪-actin鐨勪簩鎶楋紝 37鈩冩潯浠朵笅鏀剧疆1h锛汓/p>

6銆佺敤PBS鍐叉礂3娆★紝姣忔10min锛屾渶鍚庡湪鍊掔疆鑽у厜鏄惧井闀滀笅瑙傚療鍥惧儚骞舵媿鐓с€侟br/>

ATP渚濊禆鐨凴NA瑙ｆ棆閰禔娌夋穩娉昺iRNA鍒嗙璇曞墏鐩扅span style="white-space: pre;">miRNA Isolation KitMICA 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

B7-1涓€绔欏紡鏌卞紡miRNAoutOne-Stop Column miRNAOUT浜 Serum Albumin / HSA / ALB 鎶楅珨 (FITC), 鍏斿柈鎶桙/p>

B7鍚岀郴鐗?鍑濊兌娉昺iRNA鍒嗙璇曞墏鐩CD86 / B7-2 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

B7鍚屾簮浣?涓€绔欏紡澶ф彁鏌卞紡miRNAoutMaxi Column miRNAOUTCEACAM3 / CD66d 鎶楅珨 (PE), 榧犲柈鎶桙/p>

B7鍚屾簮浣?涓€绔欏紡鍔ㄧ墿mRNAoutOne-Stop Animal mRNA Isolation KitRELA / Transcription factor p65 / NFkB p65 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

B7鍚屾簮浣?涓€绔欏紡纾佺彔娉曞姩鐗﹎RNAoutOne-Step MAG Animal mRNA Isolation KitCD48 / SLAMF2 鎶楅珨 (FITC), 鍏斿柈鎶桙/p>

Bcl-2鐩稿叧X铔嬬櫧涓€绔欏紡鍏ㄨmRNAoutOne-Stop Blood mRNA Isolation KitCD48 / SLAMF2 鎶楅珨 (PE), 鍏斿柈鎶桙/p>

BH3缁撴瀯鍩熷噵浜¤瀵艰泲鐧键span style="white-space:pre">涓€绔欏紡纾佺彔娉曞叏琛€mRNA鎻愬彇璇曞墏鐩扅span style="white-space:pre">CD40 / TNFRSF5 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

Bmi-1鍩哄洜涓€绔欏紡妞嶇墿mRNAoutOne-Stop Plant mRNA Isolation KitTie2 / CD202b / TEK 鎶楅珨 (PE), 榧犲柈鎶桙/p>

BPDE-DNA鍔犲悎鐗?span style="white-space:pre">涓€绔欏紡纾佺彔娉曟鐗﹎RNAoutCD86 / B7-2 鎶楅珨 (PE), 榧犲柈鎶桙/p>

Brevican涓€绔欏紡鐪熻弻mRNAoutOne-Stop Fungal mRNA Isolation KitTie2 / CD202b / TEK 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

B绫绘竻閬撳か鍙椾綋1涓€绔欏紡纾佺彔娉曠湡鑿宮RNA鎻愬彇璇曞墏鐩扅span style="white-space:pre">CEACAM6 / CD66c 鎶楅珨 (FITC), 鍏斿柈鎶桙/p>

B-娣嬪反缁嗚優瓒嬪寲鍥犲瓙1mRNA鎻愬彇璇曞墏鐩扅span style="white-space:pre">mRNA Isolation KitP4HB 鎶楅珨 (FITC), 鍏斿柈鎶桙/p>

B缁嗚優娲诲寲鍥犲瓙纾佺彔娉昺RNA鎻愬彇璇曞墏鐩扅span style="white-space:pre">CEACAM8 / CD66b / CD67 鎶楅珨 (APC), 榧犲柈鎶桙/p>

B缁嗚優娣嬪反鐦?XLOligo锛坉T锛夌氦缁寸礌Oligo (dT) CelluloseCEACAM8 / CD66b / CD67 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

B缁嗚優娣嬪反鐦ゅ洜瀛?Oligo锛坉T锛夊啀鐢熸憾娑秉span style="white-space:pre">Oligo (dT) RechargerCD97 鎶楅珨 (PE), 鍏斿柈鎶桙/p>

B缁嗚優娣嬪反鐦ゅ洜瀛?鐢熺墿绱犳爣璁扮殑Oligo锛坉T锛堻span style="white-space:pre">Biotinylated Oligo (dT)CD97 鎶楅珨 (FITC), 鍏斿柈鎶桙/p>

C1q鑲跨槫鍧忔鍥犲瓙鐩稿叧铔嬬櫧1閾鹃湁浜插拰绱犵鐝燑span style="white-space:pre">Magnetic beads,StreptavidinCLEC4A / CLECSF6 / DCIR 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

Ca-ATP閰迭span style="white-space:pre">鍥虹浉RNase娓呴櫎鍓侟span style="white-space:pre">Surface RNase ErasolCD97 鎶楅珨 (PE), 榧犲柈鎶桙/p>

cAMP鍙嶅簲鍏冧欢缁撳悎铔嬬櫧鍥虹浉RNase娓呴櫎鍓侟span style="white-space:pre">Surface RNase ErasolS100A9 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

CC瓒嬪寲鍥犲瓙鍙椾綋1姘旂浉RNase娓呴櫎鍓侟span style="white-space:pre">Air RNase ErasolCD97 鎶楅珨 (FITC), 榧犲柈鎶桙/p>

Lactose TTC agarTTC涔崇硸鐞艰剛250g

MI MediumMI鍩瑰吇鍩裹span style="white-space:pre">1000ml

Neutral Red-bile Salt Peptone Glucose Medium缁撴櫠绱腑鎬х孩鑳嗙洂鑲夋堡250g

Coliform Medium锛圝apan锛堻span style="white-space:pre">澶ц偁鑿岀兢鍩瑰吇鍩猴紙鏃ユ湰锛堻span style="white-space:pre">250g

鑹炬牸LT100鑲夋堡250g

Nutrient Broth锛圝apan锛堻span style="white-space:pre">钀ュ吇鑲夋堡锛堟棩鏈爣鍑嗭級250g

SOC MediumSOC鍩瑰吇鍩裹span style="white-space:pre">250g

Aliz-gal Broth鑼滅礌-尾-鍗婁钩绯栬嫹鑲夋堡锛圓liz-gal鑲夋堡锛堻span style="white-space:pre">100g

Nutrient Agar锛圓gar 20g锛堻span style="white-space:pre">钀ュ吇鐞艰剛锛堢惣鑴?0g锛堻span style="white-space:pre">250g

Methyl Red Voges Proskauer Broth缂撳啿钁¤悇绯栬泲鐧借儴姘达紙MR-VP璇曢獙鐢ㄥ煿鍏诲熀锛堻span style="white-space:pre">250g

DCLS Agar鍘绘哀鑳嗛吀鐩愭灨姗奸吀鐩愪钩绯栬敆绯栫惣鑴侟span style="white-space:pre">250g

Honda Toxin-Producing BrothHonda姘忎骇姣掕倝姹｜span style="white-space:pre">250g

PSB锛堢７閰哥洂缂撳啿娑瞤H7.2锛堻span style="white-space:pre">9ml*20鏀?鍖匋/p>

Modified Sclohtens' BrothModifiedSclohtens'Broth(MSB)250g

Dextrose Broth钁¤悇绯栬倝姹｜span style="white-space:pre">250g

the Smallest Broth Medium鏈€灏忚倝姹ゅ煿鍏诲熀250g

HB-PET Auto-Indection MediumHB-PET鑷瀵煎煿鍏诲熀250g

M-Endo Mediumm-杩滆棨姘忓煿鍏诲熀250g

CAYE MediumCAYE鍩瑰吇鍩裹span style="white-space:pre">250g

Medium 鈪?for Transferring the Test Organism)鎺ョ娴嬭瘯寰敓鐗╃惣鑴傚煿鍏诲熀250g

Medium I(foe Seed and Basal Layer)鑿岀鍜屽簳灞傜惣鑴傚煿鍏诲熀250g

Inorganic Salt Medium鏃犳満鐩愬煿鍏诲熀250g

Sucrose Tryptone Broth钄楃硸鑳拌泲鐧借儴鑲夋堡200

鍙屾枡鏈堟鍩虹～閰哥洂鑳拌泲鐧借儴鑲夋堡锛圠ST锛堻span style="white-space:pre">250g

Disinfectant Neutralization Broth娑堟瘨娑蹭腑鍜岃倝姹｜span style="white-space:pre">250g

Lecithin polysorbate(LP)diluent鍗电７鑴傚悙娓╃█閲婃恫250g

A-1 MediumA-1鍩瑰吇鍩裹span style="white-space:pre">250g

M-Endo BrothM-Endo鑲夋堡250g

CCFA Agar BaseCCFA鐞艰剛鍩虹250g

Double Lactose Bile Medium鍙屾枡涔崇硸鑳嗙洂鍙戦叺鍩瑰吇鍩裹span style="white-space:pre">250g

Mossel-Enterobacteriaceae Enrichment Broth鑲犻亾鑿屽鑿岃倝姹｜span style="white-space:pre">250g

MSA MediumMSA鍩瑰吇鍩裹span style="white-space:pre">250g

Agar Medium F(Violet Red Bile Glucose Agar)鐞艰剛鍩瑰吇鍩篎(绱孩鑳嗙洂钁¤悇绯栫惣鑴?250g

BIU87/ADR浜鸿唨鑳辩檶闃块湁绱犺€愯嵂鏍?/span>Enterobacteria Enrichment Broth-Mossel鑲犻亾鑿屽瘜闆嗚倝姹ゅ煿鍏诲熀锛圲SP锛堻span style="white-space:pre">250g

Gram Negative Bacteria Enrichment Broth闈╁叞姘忛槾鎬ц弻澧炶弻娑秉span style="white-space:pre">250g

Coliphage MS2 Liquid Medium澶ц偁鏉嗚弻鍣弻浣揗S2娑蹭綋鍩瑰吇鍩裹span style="white-space:pre">250g

Coliphage MS2 Medium(Semisolid)澶ц偁鏉嗚弻鍣弻浣揗S2鍗婂浐浣撳煿鍏诲熀250g

GAD MediumGAD鍩瑰吇鍩裹span style="white-space:pre">250g

Enterobacter Cloacae Isolation Agar闃存矡鑲犳潌鑿屽垎绂荤惣鑴傦紙ECIA锛堻span style="white-space:pre">250g