璁㈣揣鍙饵/td> | 绾害瑙勬牸 | 鍖呰 | 浠锋牸(鍏? |
R2041 | 30 ml | - | |
R2042 | 100 ml | - |
鍝佺墝: | 涓滅洓 |
浜у湴: | 骞垮窞 |
鈼忎繚 瀛橈細4鈩傸br/>鈼忓埗鍝佽鏄嶞br/>鐒︾⒊閰镐簩涔欓叝锛圖iethylpyrocarbonate锛汥EPC锛夋槸涓€绉嶉珮鏁堢兎鍖栧墏锛屽彲浠ョ牬鍧廟Nase娲绘€э紝鍦ㄥ垎瀛愮敓鐗╁瀹為獙涓箍娉涘簲鐢ㄤ簬鍘婚櫎RNase鐨勬薄鏌撱€傛湰鍒跺搧鏄妸鍘荤瀛愭按缁?.1% DEPC澶勭悊鍚庯紝鍐嶇粡楂樻俯楂樺帇鐏弻鑰屽埗鎴愩€傚彲骞挎硾搴旂敤浜庡悇绉峈NA鐩稿叧瀹為獙銆侟br/>鈼忕函 搴?br/>鈼 鏈埗鍝佺粡浣撳杞綍鍙嶅簲纭鏃燫Nase姹℃煋銆侟br/>鈼 鏈埗鍝佺粡RT-PCR鍙嶅簲纭涓嶅惈瀵筊T-PCR鍙嶅簲鐨勯樆瀹崇墿銆侟br/>鈼 Nicking娲绘€ф娴婞br/> 灏? g pBR322 DNA涓庝娇鐢ㄦ湰鍒跺搧閰嶅埗鐨?0 渭l婧舵恫锛?3 mM Tris-Acetate(pH7.9)锛?0 mM閱嬮吀闀侊紝66 mM閱嬮吀閽撅級37鈩冧繚娓?灏忔椂锛屾湭妫€鍑篘icking娲绘€с€侟br/>鈼 DNase娲绘€ф娴婞br/> 灏? pmol32P鏍囪鐨剆sDNA浠ュ強dsDNA涓庝娇鐢ㄦ湰鍒跺搧閰嶅埗鐨凞Nase鍙嶅簲Buffer 10 渭l浜?7鈩冨強55鈩冨垎鍒繚娓?6灏忔椂锛屾湭妫€鍑篋Nase娲绘€с€侟br/>鈼 RNase娲绘€ф娴婞br/> 灏? g RNA涓庝娇鐢ㄦ湰鍒跺搧閰嶅埗鐨凚uffer锛?3 mM Tris- Acetate(pH7.9)锛?0 mM閱嬮吀闀侊紝66 mM閱嬮吀閽撅級37鈩冧繚娓?灏忔椂锛屾湭妫€鍑篟Nase娲绘€с€侟br/>鈼忎娇鐢ㄦ敞鎰廃br/>鏈埗鍝佺粡楂樻俯楂樺帇鐏弻鍚庢憾娑蹭腑宸蹭笉鍚獶EPC锛屼娇鐢ㄦ椂搴旈伩鍏峈Nase姹℃煋銆侟/span>