涓滅洓鐢熺墿锛圖ongsheng Biotech锛堻/div>
Guangzhou Dongsheng Biotech Co., Ltd.
鍏嶈垂浼氬憳绗?span style="font-size:1.5em;color:#f21">10骞滁/div>
鍚嶇О锛欬span class="color3 fontb">涓滅洓鐢熺墿锛圖ongsheng Biotech锛堻/span>
璁よ瘉锛欬span class="color3">宸ュ晢淇℃伅宸叉牳瀹濣/span>
鑱旂郴浜猴細 閿€鍞儴
閭紪锛欬span class="color3">510507
璁よ瘉锛欬span class="color3">宸ュ晢淇℃伅宸叉牳瀹濣/span>
鑱旂郴浜猴細 閿€鍞儴
閭紪锛欬span class="color3">510507
Random primer(50 uM)
鏍搁吀绾寲 >> RNA鎻愬彇绾寲
鍖呰涓庝环鏍硷細
| 璁㈣揣鍙饵/td> | 绾害瑙勬牸 | 鍖呰 | 浠锋牸(鍏? |
| R2031 | 50uM | 20 渭l | - |
| 鍝佺墝: | 涓滅洓 |
| 浜у湴: | 骞垮窞 |
| 浜у搧鍒悕: | Random Primer (6mer) |
浜у搧浠嬬粛
鍒跺搧淇濆瓨锛欬br/>-20鈩傸br/>鍒跺搧璇存槑锛欬br/>鍚湁6涓⒈鍩虹殑闅忔満搴忓垪寮曠墿锛堟湁46绉嶅彲鑳藉簭鍒楋級銆?鈥欐湯绔７閰稿寲淇グ銆侟br/>褰?nbsp; 鎬侊細
鏃犺壊娑蹭綋
鍒堕€犳柟娉曪細
DNA鍥虹浉鍚堟垚銆侟br/>璐ㄩ噺鏍囧噯锛欬br/>1. 鐢℉PLC妫€娴嬬函搴︺€侟br/> 2. 1 nmol鐨勬湰鍒跺搧涓? 渭g鐨?S rRNA鍦?7鈩冩潯浠朵笅鍙嶅簲24灏忔椂锛孯NA鐨勭數娉冲浘鍍忎笉鍙戠敓鍙樺寲銆?nbsp;
浣跨敤娉ㄦ剰锛欬br/>鏈埗鍝佺敤TE Buffer鎴栫伃鑿屾按婧惰В鍚庡簲閬垮厤鍙嶅鍐昏瀺銆侟br/>鐢?nbsp; 閫擖br/>1. 鐢眒RNA鍚堟垚cDNA鏃讹紝鍙唬鏇縊ligo dT鍙婂叾瀹冨紩鐗╄繘琛屽弽杞綍鍙嶅簲銆侟br/> 2. 杩涜DNA鏍囪銆侟/span>