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鑽у厜鏌撴枡灏忕粨
2021.12.23 鐐瑰嚮2092娆狘/div>

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鍏嶇柅鑽у厜 (IF)

鍦ㄨ崸鍏夋樉寰暅鎶€鏈腑锛屽彲浠ラ€氳繃涓ょ鏂瑰紡瑙傚療鍒颁綘鐨勭洰鐨勮泲鐧斤細鍒╃敤鍐呮簮鑽у厜淇″彿锛屽嵆閫氳繃鍏嬮殕鎵嬫锛岀敤閬椾紶瀛︽柟娉曞皢鑽у厜铔嬬櫧涓庣洰鐨勮泲鐧界浉杩烇紱鎴栧埄鐢ㄨ崸鍏夋爣璁扮殑鎶椾綋鐗瑰紓鎬х粨鍚堢洰鐨勮泲鐧姐€傛湁浜涚敓鐗╁闂閲囩敤绗簩绉嶆柟娉曚細鏇存湁鐢ㄦ垨鏇存湁蹇呰銆傛瘮濡傦紝缁勭粐瀛︽牱鍝佹棤娉曚娇鐢ㄨ崸鍏夎泲鐧斤紝鍥犱负閫氬父鏉ヨ锛屾爣鏈兘鏄粠鏃犳硶淇濆瓨鑽у厜铔嬬櫧鐨勭敓鐗╀綋涓幏鍙栥€傛澶栵紝褰撴湁涓€涓湁鍔熻兘鐨勬姉浣撳彲鐢ㄦ椂锛屽厤鐤崸鍏夋硶浼氭瘮鑽у厜铔嬬櫧鎶€鏈揩寰堝锛屽洜涓哄悗鑰呭繀椤诲厛鍏嬮殕鐩殑鍩哄洜鍐嶅皢DNA杞煋鍒伴€傚綋鐨勭粏鑳炰腑銆傝崸鍏夎泲鐧界殑鍙︿竴椤瑰姡鍔垮湪浜庡叾鏈韩灞炰簬铔嬬櫧璐ㄣ€傚洜姝わ紝缁嗚優鍐呯殑杩欎簺鑽у厜铔嬬櫧鍏锋湁鐗瑰畾鐨勮泲鐧借川鐗规€э紝鍏朵細瀵艰嚧闄勭潃鐨勭洰鐨勮泲鐧借川鍙戠敓鍔熻兘绱婁贡鎴栧嚭鐜拌閲婄殑鎯呭喌銆傜劧鑰岋紝鑽у厜铔嬬櫧鎶€鏈粛鐒舵槸瑙傚療娲荤粏鑳炵殑棣栭€夋柟娉曘€侟/p>

鍏嶇柅鑽у厜娉曞埄鐢ㄤ簡鎶椾綋鍙互鍜岀浉搴旀姉鍘熺壒寮傛€х粨鍚堢殑杩欎釜鐗规€э紝瀵规瀹冭繕鏈変袱绉嶄笉鍚岀殑琛ㄧ幇褰㈠紡銆傛渶绠€鍗曠殑鏂瑰紡鏄娇鐢ㄥ彲涓庣洰鐨勮泲鐧界浉缁撳悎鐨勮崸鍏夋爣璁版姉浣撱€傝繖绉嶆柟娉曡绉颁负鈥滅洿鎺ュ厤鐤崸鍏夋硶鈥濄€侟/p>

鍦ㄥ緢澶氭儏鍐典笅锛屾垜浠彲浠ュ埄鐢ㄤ袱绉嶄笉鍚岀壒鎬х殑鎶椾綋銆傜涓€绉嶆姉浣撳彲浠ョ粨鍚堢洰鐨勮泲鐧斤紝浣嗗叾鏈韩骞舵湭杩涜鑽у厜鏍囪(涓€鎶?銆傜浜岀鎶椾綋鏈韩灏辨惡甯﹁崸鍏夋煋鏂?浜屾姉)锛屽苟涓斿彲浠ョ壒寮傛€х粨鍚堜竴鎶椼€傝繖绉嶆柟娉曡绉颁负鈥滈棿鎺ュ厤鐤崸鍏夋硶鈥濄€傝繖绉嶆柟娉曞瓨鍦ㄨ澶氫紭鍔裤€備竴鏂归潰锛屽畠浼氫骇鐢熸斁澶ф晥搴旓紝鍥犱负涓嶅彧涓€涓簩鎶楀彲浠ヤ笌涓€鎶楃浉缁撳悎銆傚彟涓€鏂归潰锛屾病鏈夊繀瑕佸缁堢敤鑽у厜鏌撴枡鏍囪鐩殑铔嬬櫧鐨勬瘡涓姉浣擄紝浣嗗彲浠ヤ娇鐢ㄥ競鍞崸鍏夋爣璁扮殑浜屾姉銆傚厤鐤崸鍏変腑骞挎硾浣跨敤鐨勮崸鍏夋煋鏂欏寘鎷 FITC銆乀RITC 鎴栦竴浜汚lexa Fluor?鏌撴枡锛屼笅鏂囧潎鏈夋彁鍙娿€侟/p>

FITC 鍜 TRITC

寮傜~姘伴吀鑽у厜绱?FITC) 鏄竴绉嶆湁鏈鸿崸鍏夋煋鏂欙紝鐩墠锛岃繖绉嶈崸鍏夋煋鏂欎粛鐢ㄤ簬鍏嶇柅鑽у厜鍜屾祦寮忕粏鑳炴湳涓€傚湪 495/517 nm 澶勶紝璇ユ煋鏂欎細浜х敓婵€鍙?鍙戝皠宄板€硷紝骞跺彲鍊熷姪寮傜~姘伴吀鐩愬弽搴斿熀鍥笌涓嶅悓鎶椾綋缁撳悎锛岃鍩哄洟鍙互鍜岃泲鐧借川涓婄殑姘ㄥ熀銆佸矾鍩恒€佸挭鍞戙€侀叒姘ㄩ叞銆佺景鍩虹瓑鍩哄洟鐩哥粨鍚堛€傝€屽畠鐨勫熀鏈垚鍒嗏€斺€ 鑽у厜绱狅紝鍏舵懇灏旇川閲忎负 332 g/mol锛屽父琚敤浣滆崸鍏夌ず韪墏銆侳ITC(389 g/mol) 鏄敤浜庤崸鍏夋樉寰暅鎶€鏈殑棣栨壒鏌撴枡锛屼笖鍏惰褰撴垚 Alexa Fluor?488 绛夊悗缁崸鍏夋煋鏂欑殑鍙戠銆傝鏌撴枡鐨勮崸鍏夋椿鎬у彇鍐充簬瀹冪殑澶у叡杞姵棣欑數瀛愮郴缁燂紝鑰岃绯荤粺鍙楄摑鑹插厜璋变腑鐨勫厜鎵€婵€鍙戙€侟/p>

缁忓父涓 FITC 鍚屾椂浣跨敤鐨勫彟涓€绉嶆煋鏂欐槸涓庡叾鐩镐技鐨凾RITC [鍥涚敳鍩虹綏涓规槑-5(6)-寮傜~姘伴吀]銆備笌 FITC 鐩稿弽锛孴RITC 骞堕潪鑽у厜绱狅紝鑰屾槸缃椾腹鏄庡鏃忕殑琛嶇敓鐗┿€傜綏涓规槑涔熷叿鏈変竴涓ぇ鐨勫叡杞姵棣欑數瀛愮郴缁燂紝姝f槸璇ョ郴缁熷紩鍙戜簡瀹冧滑鐨勮崸鍏夎涓恒€傝繕鏈変竴鐐逛笌FITC 鐩稿弽锛孴RITC (479 g/mol) 鐢辨渶澶ф尝闀夸负 550nm鐨勭豢鑹插厜璋变腑鐨勫厜鎵€婵€鍙戯紝瀹冪殑鏈€澶у彂灏勬尝闀夸负 573 nm銆備笌铔嬬櫧璐?渚嬪锛屾姉浣?缁撳悎涔熷熀浜庡紓纭鞍閰哥洂鍙嶅簲鍩哄洟銆侟/p>

铏界劧 FITC 鍜 TRITC 浠嶅湪浣跨敤锛屼絾鐢变簬瀹冧滑灞炰簬鍙戝厜鐩稿杈冨急鐨勮崸鍏夋煋鏂欎笖瀹冧滑鐨勪紭鍔夸粎浠呮槸缁忔祹瀹炴儬锛屽洜姝わ紝鍦ㄦ渶鏂扮殑鏄惧井闀滄妧鏈腑骞朵笉鎺ㄨ崘銆侟/p>

闈掕壊绱燑/strong>

杩欑被鑽у厜鏌撴枡鐩稿杈冨皯锛屼粠闈掕壊绱犺鐢熻€屾潵锛屼篃鏄叾鍚嶇О鐨勭敱鏉ワ細Cy2銆丆y3銆丆y5 鍜孋y7銆備笂杩版墍鏈夐潚鑹茬礌鍧囧彲浠ラ€氳繃鍏跺弽搴斿熀鍥笌鏍搁吀鎴栬泲鐧界浉杩炪€備緥濡傦紝閲囩敤浜嗚泲鐧芥爣璁扮殑椹潵閰颁簹鑳哄熀鍥€€傛湁瓒g殑鏄紝瀵逛簬鑽у厜锛孋y5 瀵瑰叾鍛ㄨ竟鐢靛瓙鐜闈炲父鏁忔劅锛岃鐗瑰緛鍙敤浜庨叾娴嬪畾銆傞檮鐫€铔嬬櫧璐ㄧ殑鏋勮薄鏀瑰彉浼氬鑷磋崸鍏夊彂灏勪骇鐢熼槼鎬ф垨闃存€у彉鍖栥€傛澶栵紝Cy3 鍜 Cy5 杩樺彲鐢ㄤ簬 FRET 璇曢獙銆傞潚鑹茬礌鏌撴枡鏄竴绉嶇浉瀵硅緝鑰佺殑鑽у厜鏌撴枡锛屼絾鍗存槸鍏朵粬鑽у厜鏌撴枡鍦ㄤ寒搴︺€佽€愬厜鎬с€侀噺瀛愪骇鐜囩瓑鏂归潰寰椾互鏀瑰杽鐨勫熀纭€銆侟/p>

Alexa Fluor?鏌撴枡

Alexa Fluor?鏌撴枡鏄甫璐熺數鑽蜂笖浜叉按鐨勮崸鍏夋煋鏂欑郴鍒楋紝璇ョ郴鍒楁煋鏂欏泭鎷寖鍥磋緝骞匡紝涓旂粡甯哥敤浜庤崸鍏夋樉寰暅鎶€鏈箣涓€傝繖浜涙煋鏂欑殑鍚嶇О鏄敱鍏跺彂鏄庤€匯ichard Paul Haugland 浠ヤ粬鍎垮瓙 Alex Haugland 鐨勫悕瀛楀懡鍚嶇殑銆傝浜у搧鏍囪瘑鏄 Molecular Probes(缇庡浗鐢熷懡绉戝鎶€鏈叕鍙 Life Technologies鏃椾笅瀛愬叕鍙革紝娉細2014骞?鏈圠ife Technologies琚玊hermo Fisher鏀惰喘)鐨勫晢鏍囥€傛澶栵紝杩欎簺浜у搧鏍囪瘑涓篃娑电洊浜嗙浉搴旂殑婵€鍏夋縺鍙戞尝闀裤€備緥濡傦紝搴旂敤鑼冨洿寰堝箍涓旀渶澶ф縺鍙戞尝闀夸负 493 nm鐨凙lexa Fluor?488锛屽彲鐢辨爣鍑嗙殑488 nm婵€鍏夋縺鍙戙€侫lexa Fluor?488鐨勬渶澶у彂灏勬尝闀夸负 519 nm锛屾鏄洜涓哄叿澶囦笂杩扮壒鎬э紝浣垮緱 Alexa Fluor?488涓 FITC 鐨勫睘鎬х浉浼笺€傚敖绠 Alexa Fluor?488鏄竴绉嶈崸鍏夌礌琛嶇敓鐗╋紝浣嗕笌 FITC 鐩稿弽锛屽畠鎷ユ湁鏇翠匠鐨勭ǔ瀹氭€у拰鑽у厜浜害锛屼笖 pH 鏁忔劅搴︿篃鏇翠綆銆傛墍鏈 Alexa Fluor? 鏌撴枡(姣斿锛孉lexa Fluor?546銆丄lexa Fluor?633)閮芥槸涓嶅悓鍩虹鑽у厜鐗╄川鐨勭:鍖栧舰寮忥紝渚嬪锛岃崸鍏夌礌銆侀璞嗙礌銆侀潚鑹茬礌鎴栫綏涓规槑锛屽畠浠殑鎽╁皵璐ㄩ噺鍦?10 鑷 1400 g/mol 鑼冨洿涔嬪唴銆侟/p>

DNA 鏌撹壊

鍦ㄨ崸鍏夋樉寰暅鎶€鏈腑锛屼笉鍙爺绌惰泲鐧界粨鏋勶紝鏍搁吀鍚屾牱鍏锋湁閲嶈鐨勭爺绌舵剰涔夈€傛湁鏃跺€欙紝蹇呴』閫氳繃妫€娴嬬粏鑳炴牳鏉ョ‘瀹氱粏鑳炵殑绮剧‘浣嶇疆鍙婂叾鏁伴噺銆傛渶甯哥敤鐨勪竴绉岲NA 鏌撹壊鍓傚綋灞 DAPI (4',6-浜岃剴鍩?2-鑻熀鍚插摎) 锛屽叾鍙笌DNA 鍙岃灪鏃嬬殑 A-T 瀵岄泦鍖哄煙鐩哥粨鍚堛€傚鏋 DAPI 闄勭潃鍒 DNA 涓婏紝鍏惰崸鍏夊己搴﹀皢姣旀父绂荤姸鎬佽楂樸€傝鏌撹壊鍓傚彈鏈€澶ф尝闀夸负358 nm鐨勭传澶栧厜婵€鍙戯紝鍏跺彂灏勫厜璋遍潪甯稿锛屽湪461 nm 澶勮揪鍒板嘲鍊笺€傛澶栵紝杩樺彲瀵瑰急鑽у厜杩涜 RNA 缁撳悎妫€娴嬨€傚湪杩欑鎯呭喌涓嬶紝鍙戝皠娉㈤暱灏嗚浆绉昏嚦 500 nm銆傛湁瓒g殑鏄紝DAPI 鑳藉绌块€忔暣涓粏鑳炶啘銆傚洜姝わ紝瀹冨彲浠ョ敤浜庡浐瀹氬拰娲荤粏鑳炰箣涓€侟/p>

绗簩绉嶈骞挎硾浣跨敤鐨凞NA 鏌撹壊鍓傚氨鏄 Hoechst 鏌撴枡绯诲垪锛岃繖浜涙煋鏂欏師鍏堥兘鏄敱 Hoechst AG 杩欏鍖栧鍏徃鐢熶骇鐨勩€侶oechst 33258銆丠oechst 33342浠ュ強Hoechst 34580 鍧囦负鍙岃嫰閰颁簹鑳猴紝鍙祵鍏 A-T 瀵岄泦鍖哄煙锛屽洜姝わ紝璇ョ郴鍒楁煋鏂欏緢灏戠敤鍒般€備笌 DAPI 鐩镐技锛岃繖涓夌鏌撹壊鍓傞兘鍙彈鏈€澶у彂灏勬尝闀夸负455 nm鐨勭传澶栧厜婵€鍙戯紝鑰屾湭琚粨鍚堢殑鏌撹壊鍓傦紝鍏舵渶澶у彂灏勬尝闀垮湪 510鈥?40 nm 涔嬮棿銆侶oechst 鏌撹壊鍓傝繕鍏锋湁缁嗚優娓楅€忔€э紝鍥犳鍙敤浜庢椿缁嗚優鎴栧凡鍥哄畾鐨勭粏鑳炰腑銆傝鏌撹壊鍓備笌DAPI 鐨勪笉鍚屼箣澶勫湪浜庯紝瀹冧滑鐨勬瘨鎬ц緝浣庛€侟/p>

纰樺寲涓欏暥(Propidium-Iodide锛孭I)鏄竴绉嶄笉鑳介€忚繃缁嗚優鑶滅殑 DNA 鏌撹壊鍓傘€傜敱浜庡叿澶囦笂杩扮壒鎬э紝璇ユ煋鑹插墏鏃犳硶杩涘叆瀹屾暣鐨勭粏鑳炰腑锛屽洜姝わ紝璇ユ煋鑹插墏甯哥敤浜庡尯鍒嗙粏鑳炵兢涓殑娲荤粏鑳炲拰姝荤粏鑳炪€傛澶栵紝纰樺寲涓欏暥杩樻槸涓€绉嶅祵鍏ュ墏锛屼絾瀵逛簬涓嶅悓鐨勭⒈鍩哄苟涓嶅瓨鍦ㄧ粨鍚堢殑宸紓鎬с€傝鏌撹壊鍓備笌鏍搁吀缁撳悎鍚庯紝鏈€澶ф縺鍙戞尝闀夸负538 nm锛屾渶澶у彂灏勬尝闀夸负 617 nm銆傛湭缁撳悎 PI 鐨勬渶澶ф縺鍙戝拰鍙戝皠娉㈤暱鍜屽厜寮轰細鏇翠綆涓€浜涖€侾I 杩樺彲缁撳悎 RNA锛屽悓鏃舵棤闇€鏀瑰彉鑷韩鐨勮崸鍏夌壒鎬с€傛湁鏃跺€欎负浜嗗尯鍒咲NA 鍜 RNA锛屾湁蹇呰浣跨敤閫傚綋鐨勬牳閰搁叾銆侟/p>

涓嶉渶瑕佸墠鏈熷鐞嗭紝鍚栧暥姗欏氨鍙互閴村埆DNA 涓 RNA 銆傚悥鍟舵涓 DNA 缁撳悎鍚庯紝鏈€澶ф縺鍙?鍙戝皠娉㈤暱涓 502 nm/525 nm锛岃€屼笌 RNA 缁撳悎鍚庯紝鏈€澶ф縺鍙?鍙戝皠娉㈤暱涓?60 nm/650 nm銆傛澶栵紝瀹冭繕鑳藉杩涘叆婧堕叾浣撶瓑閰告€у尯瀹ゃ€傚湪杩欓噷锛岄槼绂诲瓙鏌撴枡琚川瀛愬寲銆傚湪杩欑閰告€х幆澧冧笅锛屽悥鍟舵鐢辫摑鑹插厜璋变腑鐨勫厜婵€鍙戯紝浣嗗彂灏勬尝闀垮湪姗欒壊鍖哄煙杈惧埌鏈€澶с€傜敱浜庡噵浜$粏鑳炲叿鏈夊ぇ閲忚鍚炲櫖鐨勯吀鎬у尯瀹わ紝鍥犳锛屽悥鍟舵甯哥敤浣滄绫荤粏鑳炵殑鏍囪鐗┿€侟/p>

鍖哄鍜岀粏鑳炲櫒鐗瑰紓鎬ф煋鏂橖/strong>

鍦ㄨ崸鍏夋樉寰暅鎶€鏈腑锛屽線寰€瑕佸婧堕叾浣撱€佹牳鍐呬綋绛夌粏鑳炲尯瀹や互鍙婄嚎绮掍綋绛夌粏鑳炲櫒杩涜鏌撹壊銆備负姝わ紝璇ラ儴鍒嗕粙缁嶄簡涓€绯诲垪鍙緵閫夋嫨浣跨敤鐨勭壒寮傛€ф煋鏂欍€侟/p>

瑙傚療绾跨矑浣撴渶甯哥敤鐨勬柟娉曞氨鏄埄鐢 MitoTracker?锛屽畠鏄竴绉嶅彲閫忚繃缁嗚優鐨勬煋鏂欙紝鍖呭惈杞诲害宸熀鍖栫殑姘敳鍩烘椿鎬ч儴鍒嗐€傛鍥犲姝わ紝瀹冨彲涓庡崐鑳辨皑閰告畫鍩虹殑娓哥纭唶鍩哄弽搴旓紝浠庤€屼笌鍩鸿川铔嬬櫧瀹炵幇鍏变环缁撳悎銆侻itoTracker? 鏈変笉鍚岀殑棰滆壊鍜屼慨楗扮被鍨?鍙傝琛 1)锛屾澶栵紝瀹冭繕鏄 Molecular Probes 鐨勫晢鏍囥€備笌缃椾腹鏄 123 (Rh123) 鎴 tetramethylrosamine绛夊父瑙勭嚎绮掍綋鐗瑰紓鎬ф煋鑹插墏涓嶅悓锛屽湪鐢ㄥ浐瀹氬墏鐮村潖鑶滅數浣嶅悗锛孧itoTracker?涓嶄細琚礂鎺夈€侟/p>

渚濇嵁绾跨矑浣撴煋鑹插墏锛岃繕鏈変簺鏌撴枡鍙互鏍囪婧堕叾浣撶瓑閰告€у尯瀹わ紝杩欑被鏌撴枡琚О涓篖ysoTracker銆傚畠浠敱杩炴帴涓€涓崸鍏夊熀鍥㈢殑寮辩⒈鍩哄洟缁勬垚锛屽叿鏈夎啘绌块€忔€с€傛渶鏈夊彲鑳界殑鎯呭喌鏄紝杩欎簺纰卞熀鍥犺川瀛愬寲浣滅敤鐨勫奖鍝嶈€屽閰告€у尯瀹ゅ叿鏈変翰鍜屾€с€侺ysoTracker鍏锋湁澶氱涓嶅悓鐨勯鑹插彲渚涢€夋嫨(鍙傝琛 1)銆侟/p>

涓庢憾閰朵綋鐩镐技鐨勫尯瀹ゆ槸閰块厭閰垫瘝绛夌湡鑿屼腑鐨勬恫娉★紝杩欑鑶滃瘑闂┖闂翠篃鏄竴绉嶉吀鎬х幆澧冦€傚鏋滆鍦ㄨ崸鍏夋樉寰暅涓嬭瀵熶笂杩板尯瀹わ紝鍒欒浣跨敤FM 4-64?鎴朏M 5-95?绛夎嫰涔欑儻鍩烘煋鏂欍€侟/p>

瀵逛簬铔嬬櫧璐ㄥ垎娉屽疄楠岋紝鍐呰川缃 (ER) 鍏锋湁閲嶈鐨勭爺绌舵剰涔夈€傚涓婅堪鍖哄杩涜鏌撹壊鐨勪竴绉嶅吀鍨嬫煋鏂欎负DiOC6(3)銆傝鏌撴枡铏界劧鍋忓ソ ER锛屼絾浠嶄細缁撳悎绾跨矑浣撶瓑鍏朵粬缁嗚優鍣ㄨ啘銆傚ER 杩涜鐗瑰紓鎬ф煋鑹茬殑鍙︿竴绉嶆柟娉曟槸锛屼娇鐢 ER-Tracker Green 鍜 Red 绛 ER-Tracker锛岃€ ER-Tracker Green 鍜 Red 鏄熀浜 BODIPY 鐨勪袱绉嶆煋鏂欙紝鍏朵笌鏍煎垪鏈劜(涓€绉嶇:閰板熀鑴查叾)杩炴帴锛屽苟鍙笌浠呭瓨浜庡唴璐ㄧ綉鑶滀笂鐨凙TP鏁忔劅鎬ч捑閫氶亾缁撳悎銆侭ODIPY(纭?浜屽悺鍜簹鐢插熀锛宐oron-dipyrromethene)鏄竴绉嶅嚑涔庝笉婧朵簬姘寸殑銆佸pH 鐩稿涓嶆晱鎰熺殑鏌撴枡鍩哄洟锛岃鏌撴枡瀵硅泲鐧借川鏍囪娌″お澶х敤澶勶紝浣嗗嵈鏄剛璐ㄥ拰鑶滄爣璁扮殑鑹ソ宸ュ叿銆侟/p>

瀵逛簬涓嶦R鐩搁偦鐨勯珮灏斿熀浣擄紝鍙互鐢 NBD C6-ceramide 鍜孊ODIPYFL C5-ceramide 绛夎崸鍏夌缁忛叞鑳虹被浼肩墿瀵瑰叾杩涜鏍囪銆 涓婅堪绁炵粡閰拌兒涓洪灅鑴傜被锛屽叾鍦ㄩ珮灏斿熀浣撲腑楂樺害瀵岄泦銆侟/p>

鍊熷姪鍩轰簬鑴傝川鐨勬煋鏂欙紝鍙互瀵硅剛绛忕瓑鐗瑰紓鑶滃尯鍩熻繘琛屾煋鑹层€備娇鐢 NBD-6Cholestrol鎴朜BP-12 Cholesterol 鍙互瑙傚療鑳嗗浐閱囧瘜闆嗗尯鍩?Avanti Polar Lipids)銆侟/p>

闄や簡浣跨敤鐗瑰紓鎬ч潪铔嬬櫧鑽у厜鏌撴枡瀵圭粏鑳炲尯瀹よ繘琛屾爣璁颁箣澶栵紝杩樺彲浠ュ€熷姪瀵圭粏鑳炰腑涓嶅悓浣嶇疆鏈夊亸濂界殑铔嬬櫧璐ㄥ鐩爣鍖哄煙杩涜鏌撹壊銆傝繖浜涜泲鐧借川鍙互鍜岃崸鍏夋煋鏂欑浉杩烇紝骞跺彲閫氳繃鑽у厜鏄惧井闀滆繘琛岃瀵熴€傝繍鐢ㄨ繖绉嶆柟娉曠殑涓€涓疄渚嬫槸锛氶害鑳氬嚌闆嗙礌(WGA) 鍙互涓庣粏鑳炶川鑶滀腑鐨勫斁娑查吀鍜孨-涔欓叞钁¤悇绯栬兒鍩虹壒寮傛€х粨鍚堬紝灏哤GA 涓庤崸鍏夋煋鏂欏伓鑱旓紝杩欐牱鎴戜滑灏卞彲浠ヨ瀵熷埌缁嗚優璐ㄨ啘浜嗐€侟/p>

绂诲瓙鎴愬儚

鍦ㄦ湁鍏崇缁忓厓鏂归潰鐨勭爺绌朵腑锛岃瀵熷熀鍥犳椿鎬ф垨缁嗚優杩愬姩绛夊浜庝簡瑙g粏鑳炵殑绂诲瓙娴撳害鍏锋湁閲嶈鎰忎箟銆傞挔銆侀挋銆佹隘鎴栭晛绂诲瓙瀵瑰緢澶氫笉鍚岀殑缁嗚優娲诲姩閮藉叿鏈夎緝澶у奖鍝嶃€備竴鑸儏鍐典笅锛屽€熷姪鑽у厜鏍囪鐨勮灟鍚堝墏鍙皢绂诲瓙鍥颁綇锛岃灟鍚堝墏鍦ㄧ粨鍚堢浉搴旂瀛愬悗浼氭敼鍙樼瀛愮殑鍏夎氨鐗规€с€備緥濡傦紝鍒╃敤璇ュ師鐞嗙殑閽欐寚绀哄墏fura-2銆乮ndo-1銆乫luo-3銆乫luo-4鍜孋alcium-Green 绛夈€侟/p>

瀵逛簬閽犵瀛愮殑妫€娴嬶紝閫氬父浣跨敤 SBFI (sodium-bindingbenzofurzanisophthalate) 鎴朣odium Green銆侾BFI(potassium-binding benzofurzanisophthalate) 鍙互妫€娴嬮捑绂诲瓙銆侟/p>

鏈夎叮鐨勬槸锛岃繕瀛樺湪浠ヨ泲鐧借川涓轰富鐨勯挋鎸囩ず鍓傦紝鍏朵腑涓€绉嶆槸鍩轰簬姘存瘝鍖栧鍙戝厜铔嬬櫧鈥旀按姣嶇礌銆傛按姣嶇礌銆佸彂鍏変綋鑵旇偁绱犮€佸垎瀛愭哀鍜 Ca2+鐨勭浉浜掍綔鐢ㄤ細閲婃斁钃濆厜锛岃繖鏄湪鑽у厜铔嬬櫧璐ㄧ殑鍙戠幇杩囩▼涓潪甯歌憲鍚嶇殑鏈哄埗銆侟/p>

鑽у厜鏌撴枡鍙婂叾婵€鍙戝拰鍙戝皠娉㈤暱宄板€稽/strong>

涓婃枃鎻愬埌鐨勬墍鏈夋煋鏂欏湪涓嬭〃涓潎鏈夌粺璁°€傛澶栵紝杩樻兜鐩栦簡鍏朵粬鑽у厜鏌撴枡鍙婂叾婵€鍙戝拰鍙戝皠娉㈤暱宄板€笺€侟/p>

鑽у厜鏌撴枡绀轰緥 婵€鍙戝厜
Indo-1, Ca saturated 331 nm
Indo-1 Ca2+ 346 nm
Cascade Blue BSA pH 7.0 401 nm
Cascade Blue 398 nm
LysoTracker Blue 373 nm
Alexa 405 401 nm
LysoSensor Blue pH 5.0 374 nm
LysoSensor Blue 374 nm
DyLight 405 399 nm
DyLight 350 332 nm
BFP (Blue Fluorescent Protein) 380 nm
Alexa 350 343 nm
7-Amino-4-methylcoumarin pH 7.0 346 nm
Amino Coumarin 345 nm
AMCA conjugate 347 nm
Coumarin 360 nm
7-Hydroxy-4-methylcoumarin 360 nm
7-Hydroxy-4-methylcoumarin pH 9.0 361 nm
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0 358 nm
Hoechst 33342 352 nm
Pacific Blue 404 nm
Hoechst 33258 352 nm
Hoechst 33258-DNA 352 nm
Pacific Blue antibody conjugate pH 8.0 404 nm
PO-PRO-1 434 nm
PO-PRO-1-DNA 435 nm
POPO-1 433 nm
POPO-1-DNA 433 nm
DAPI-DNA 359 nm
DAPI 358 nm
Marina Blue 362 nm
SYTOX Blue-DNA 445 nm
CFP (Cyan Fluorescent Protein) 434 nm
eCFP (Enhanced Cyan Fluorescent Protein) 437 nm
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS) 375 nm
Indo-1, Ca free 346 nm
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid) 375 nm
BO-PRO-1-DNA 462 nm
BOPRO-1 462 nm
BOBO-1-DNA 461 nm
SYTO 45-DNA 451 nm
evoglow-Pp1 448 nm
evoglow-Bs1 448 nm
evoglow-Bs2 448 nm
Auramine O 431 nm
DiO 487 nm
LysoSensor Green pH 5.0 447 nm
Cy 2 489 nm
LysoSensor Green 447 nm
Fura-2, high Ca 336 nm
Fura-2 Ca2+sup> 336 nm
SYTO 13-DNA 488 nm
YO-PRO-1-DNA 491 nm
YOYO-1-DNA 491 nm
eGFP (Enhanced Green Fluorescent Protein) 488 nm
LysoTracker Green 503 nm
GFP (S65T) 489 nm
BODIPY FL, MeOH 502 nm
Sapphire 396 nm
BODIPY FL conjugate 503 nm
MitoTracker Green 490 nm
MitoTracker Green FM, MeOH 490 nm
Fluorescein 0.1 M NaOH 493 nm
Calcein pH 9.0 494 nm
Fluorescein pH 9.0 490 nm
Calcein 493 nm
Fura-2, no Ca 367 nm
Fluo-4 494 nm
FDA 495 nm
DTAF 495 nm
Fluorescein 495 nm
Fluorescein antibody conjugate pH 8.0 493 nm
CFDA 495 nm
FITC 495 nm
Alexa Fluor 488 hydrazide-water 493 nm
DyLight 488 493 nm
5-FAM pH 9.0 492 nm
FITC antibody conjugate pH 8.0 495 nm
Alexa 488 493 nm
Rhodamine 110 497 nm
Rhodamine 110 pH 7.0 497 nm
Acridine Orange 431 nm
Alexa Fluor 488 antibody conjugate pH 8.0 499 nm
BCECF pH 5.5 485 nm
PicoGreendsDNA quantitation reagent 502 nm
SYBR Green I 498 nm
Rhodaminen Green pH 7.0 497 nm
CyQUANT GR-DNA 502 nm
NeuroTrace 500/525, green fluorescent Nissl stain-RNA 497 nm
DansylCadaverine 335 nm
Rhodol Green antibody conjugate pH 8.0 499 nm
Fluoro-Emerald 495 nm
Nissl 497 nm
Fluorescein dextran pH 8.0 501 nm
Rhodamine Green 497 nm
5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0 504 nm
DansylCadaverine, MeOH 335 nm
eYFP (Enhanced Yellow Fluorescent Protein) 514 nm
Oregon Green 488 498 nm
Oregon Green 488 antibody conjugate pH 8.0 498 nm
Fluo-3 506 nm
BCECF pH 9.0 501 nm
SBFI-Na+ 336 nm
Fluo-3 Ca2+ 506 nm
Rhodamine 123, MeOH 507 nm
FlAsH 509 nm
Calcium Green-1 Ca2+ 506 nm
Magnesium Green 507 nm
DM-NERF pH 4.0 493 nm
Calcium Green 506 nm
Citrine 515 nm
LysoSensor Yellow pH 9.0 335 nm
TO-PRO-1-DNA 515 nm
Magnesium Green Mg2+ 507 nm
Sodium Green Na+ 507 nm
TOTO-1-DNA 514 nm
Oregon Green 514 512 nm
Oregon Green 514 antibody conjugate pH 8.0 513 nm
NBD-X 466 nm
DM-NERF pH 7.0 509 nm
NBD-X, MeOH 467 nm
CI-NERF pH 6.0 513 nm
Alexa 430 431 nm
Alexa Fluor 430 antibody conjugate pH 7.2 431 nm
CI-NERF pH 2.5 504 nm
Lucifer Yellow, CH 428 nm
LysoSensor Yellow pH 3.0 389 nm
6-TET, SE pH 9.0 521 nm
Eosin antibody conjugate pH 8.0 525 nm
Eosin 524 nm
6-Carboxyrhodamine 6G pH 7.0 526 nm
6-Carboxyrhodamine 6G, hydrochloride 525 nm
Bodipy R6G SE 528 nm
BODIPY R6G, MeOH 528 nm
6 JOE 520 nm
Cascade Yellow antibody conjugate pH 8.0 399 nm
Cascade Yellow 399 nm
mBanana 540 nm
Alexa Fluor 532 antibody conjugate pH 7.2 528 nm
Alexa 532 528 nm
Erythrosin-5-isothiocyanate pH 9.0 533 nm
6-HEX, SE pH 9.0 534 nm
mOrange 548 nm
mHoneydew 478 nm
Cy 3 549 nm
Rhodamine B 543 nm
DiI 551 nm
5-TAMRA-MeOH 543 nm
Alexa 555 553 nm
Alexa Fluor 555 antibody conjugate pH 7.2 553 nm
DyLight 549 555 nm
BODIPY TMR-X, SE 544 nm
BODIPY TMR-X, MeOH 544 nm
PO-PRO-3-DNA 539 nm
PO-PRO-3 539 nm
Rhodamine 551 nm
Bodipy TMR-X conjugate 544 nm
POPO-3 533 nm
Alexa 546 562 nm
BODIPY TMR-X antibody conjugate pH 7.2 544 nm
Calcium Orange Ca2+ 549 nm
TRITC 550 nm
Calcium Orange 549 nm
Rhodaminephalloidin pH 7.0 558 nm
MitoTracker Orange 551 nm
MitoTracker Orange, MeOH 551 nm
Phycoerythrin 565 nm
Magnesium Orange 550 nm
R-Phycoerythrin pH 7.5 565 nm
5-TAMRA pH 7.0 553 nm
5-TAMRA 549 nm
Rhod-2 552 nm
FM 1-43 472 nm
Rhod-2 Ca2+ 553 nm
Tetramethylrhodamine antibody conjugate pH 8.0 552 nm
FM 1-43 lipid 473 nm
LOLO-1-DNA 568 nm
dTomato 554 nm
DsRed 563 nm
Dapoxyl (2-aminoethyl) sulfonamide 372 nm
Tetramethylrhodamine dextran pH 7.0 555 nm
Fluor-Ruby 554 nm
Resorufin 571 nm
Resorufin pH 9.0 571 nm
mTangerine 568 nm
LysoTracker Red 578 nm
Lissaminerhodamine 572 nm
Cy 3.5 578 nm
Rhodamine Red-X antibody conjugate pH 8.0 573 nm
Sulforhodamine 101, EtOH 578 nm
JC-1 pH 8.2 593 nm
JC-1 592 nm
mStrawberry 575 nm
MitoTracker Red 578 nm
MitoTracker Red, MeOH 578 nm
X-Rhod-1 Ca2+ 580 nm
Alexa Fluor 568 antibody conjugate pH 7.2 579 nm
Alexa 568 576 nm
5-ROX pH 7.0 578 nm
5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt) 578 nm
BO-PRO-3-DNA 574 nm
BOPRO-3 574 nm
BOBO-3-DNA 570 nm
Ethidium Bromide 524 nm
ReAsH 597 nm
Calcium Crimson 589 nm
Calcium Crimson Ca2+ 590 nm
mRFP 585 nm
mCherry 587 nm
Texas Red-X antibody conjugate pH 7.2 596 nm
HcRed 590 nm
DyLight 594 592 nm
Ethidium homodimer-1-DNA 528 nm
Ethidiumhomodimer 528 nm
Propidium Iodide 538 nm
SYPRO Ruby 467 nm
Propidium Iodide-DNA 538 nm
Alexa 594 590 nm
BODIPY TR-X, SE 588 nm
BODIPY TR-X, MeOH 588 nm
BODIPY TR-X phallacidin pH 7.0 590 nm
Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2 567 nm
YO-PRO-3-DNA 613 nm
Di-8 ANEPPS 469 nm
Di-8-ANEPPS-lipid 469 nm
YOYO-3-DNA 612 nm
Nile Red-lipid 553 nm
Nile Red 559 nm
DyLight 633 624 nm
mPlum 587 nm
TO-PRO-3-DNA 642 nm
DDAO pH 9.0 648 nm
Fura Red, high Ca 434 nm
Allophycocyanin pH 7.5 651 nm
APC (allophycocyanin) 650 nm
Nile Blue, EtOH 631 nm
TOTO-3-DNA 642 nm
Cy 5 646 nm
BODIPY 650/665-X, MeOH 646 nm
Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2 569 nm
DyLight 649 652 nm
Alexa Fluor 647 antibody conjugate pH 7.2 653 nm
Alexa 647 653 nm
Fura Red Ca2+ 435 nm
Atto 647 644 nm
Fura Red, low Ca 472 nm
Carboxynaphthofluorescein pH 10.0 600 nm
Alexa 660 664 nm
Alexa Fluor 660 antibody conjugate pH 7.2 663 nm
Cy 5.5 673 nm
Alexa Fluor 680 antibody conjugate pH 7.2 679 nm
Alexa 680 679 nm
DyLight 680 678 nm
Alexa Fluor 700 antibody conjugate pH 7.2 696 nm
Alexa 700 696 nm
FM 4-64, 2% CHAPS 506 nm
FM 4-64 508 nm
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