闄愬埗鎬ф牳閰稿唴鍒囬叾鏄彲浠ヨ瘑鍒壒瀹氱殑鏍歌嫹閰稿簭鍒楋紝骞跺湪姣忔潯閾句腑鐗瑰畾閮ㄤ綅鐨勪袱涓牳鑻烽吀涔嬮棿鐨勭７閰镐簩閰敭杩涜鍒囧壊鐨勪竴绫婚叾锛岀畝绉伴檺鍒堕叾銆侟/span>

涓€鑸槸浠ュ井鐢熺墿灞炲悕鐨勭涓€涓瓧姣嶅拰绉嶅悕鐨勫墠涓や釜瀛楁瘝缁勬垚锛岀鍥涗釜瀛楁瘝琛ㄧず鑿屾牚(鍝佺郴)銆備緥濡傦紝浠嶣acillus amylolique faciens H涓彁鍙栫殑闄愬埗鎬у唴鍒囬叾绉颁负Bam H锛屽湪鍚屼竴鍝佺郴缁嗚弻涓緱鍒扮殑璇嗗埆涓嶅悓纰卞熀椤哄簭鐨勫嚑绉嶄笉鍚岀壒寮傛€х殑閰讹紝鍙互缂栨垚涓嶅悓鐨勫彿锛屽Hind鈪°€丠ind鈪紝HpaI銆丠pa鈪★紝MboI銆丮bo鈪＄瓑銆侟br/> 鍐嶅EcoRI锛欬/span>

鏍规嵁闄愬埗閰剁殑缁撴瀯锛岃緟鍥犲瓙鐨勯渶姹傚垏浣嶄笌浣滅敤鏂瑰紡锛屽彲灏嗛檺鍒堕叾鍒嗕负涓夌绫诲瀷锛屽垎鍒槸绗竴鍨?Type I)銆佺浜屽瀷(Type 鈪?鍙婄涓夊瀷(Type 鈪?銆侟br/> 1銆佺涓€鍨嬮檺鍒堕叾
鍚屾椂鍏锋湁淇グ(modification)鍙婅瘑鍒垏鍓?restriction)鐨勪綔鐢紱鍙︽湁璇嗗埆(recognize)DNA涓婄壒瀹氱⒈鍩哄簭鍒楃殑鑳藉姏锛岄€氬父鍏跺垏鍓蹭綅(cleavage site)璺濈璇嗗埆浣?recognition site)鍙揪鏁板崈涓⒈鍩轰箣杩溿€備緥濡傦細EcoB銆丒coK銆侟br/> 2銆佺浜屽瀷闄愬埗閰迭br/> 鍙叿鏈夎瘑鍒垏鍓茬殑浣滅敤锛屼慨楗颁綔鐢ㄧ敱鍏朵粬閰惰繘琛屻€傛墍璇嗗埆鐨勪綅缃涓虹煭鐨勫洖鏂囧簭鍒?palindrome sequence)锛涙墍鍓垏鐨勭⒈鍩哄簭鍒楅€氬父鍗充负鎵€璇嗗埆鐨勫簭鍒椼€傛槸閬椾紶宸ョ▼涓婏紝瀹炵敤鎬ц緝楂樼殑闄愬埗閰剁绫汇€備緥濡傦細EcoRI銆丠ind鈪€€侟br/> 3銆佺涓夊瀷闄愬埗閰迭br/> 涓庣涓€鍨嬮檺鍒堕叾绫讳技锛屽悓鏃跺叿鏈変慨楗板強璇嗗埆鍒囧壊鐨勪綔鐢ㄣ€傚彲璇嗗埆鐭殑涓嶅绉板簭鍒楋紝鍒囧壊浣嶄笌璇嗗埆搴忓垪绾﹁窛24-26涓⒈鍩哄銆備緥濡傦細Hinf鈪€€侟/span>

闄愬埗鎬ф牳閰稿唴鍒囬叾鍒嗗竷鏋佸箍锛屽嚑涔庡湪鎵€鏈夌粏鑿岀殑灞炪€佺涓兘鍙戠幇鑷冲皯涓€绉嶉檺鍒舵€у唴鍒囬叾锛屽鑰呭湪涓€灞炰腑灏辨湁鍑犲崄绉嶏紝渚嬪鍦ㄥ棞琛€鏉嗚弻灞炰腑(Haemophilus)鐜板凡鍙戠幇鐨勫氨鏈?2绉嶃€傛湁鐨勮弻鏍惈閰堕噺鏋佷綆锛屽緢闅惧垎绂诲畾鎬э紱鐒惰€屽湪鏈夌殑鑿屾牚涓紝閰跺惈閲忔瀬楂橈紟濡侲. coli鐨刾MB4(EcoRI閰?鍜孒. aegyptius(Hal 鈪㈤叾)灏辨槸楂樹骇閰惰弻鏍€傛嵁鎶ラ亾浠?0g鐨凥. aegyptius鐨勭粏鑳炰腑锛岃兘鍒嗙鎻愮函鍑哄彲娑堝寲l0g位鍣尩浣揇NA鐨勯叾閲忋€傚埌鐩墠涓烘锛岀粏鑿屾槸闄愬埗鎬у唴鍒囬叾锛屽挨鍏舵槸鐗瑰紓鎬ч潪甯稿己鐨処绫婚檺鍒舵€у唴鍒囬叾鐨勪富瑕佹潵婧愩€侟/span>