CCK-8璇曞墏鐩扅/span>

Cell Counting Kit -8

璐у彿	瑙勬牸
CT-K-5	5姣崌,500娆狘/span>
CT-K-10	10姣崌,1000娆狘/span>

妫€娴嬪師鐞咟/span>

CCK-8璇曞墏鐩掓槸涓€绉嶅熀浜嶞/span>WST-8鐨勫箍娉涘簲鐢ㄤ簬缁嗚優澧炴畺鍜岀粏鑳炴瘨鎬х殑蹇€熼珮鐏垫晱搴︽娴嬭瘯鍓傜洅銆侟/span>

WST-8灞炰簬MTT鐨勫崌绾т骇鍝侊紝鑳藉湪鐢靛瓙杞戒綋1-鐢叉哀鍩裹/span>PMS鐨勪綔鐢ㄤ笅琚繕鍘熸垚姘存憾鎬х敳鑷滀骇鐗╋紝鐢熸垚鐨勭敳鑷滈噺涓庢椿缁嗚優鏁伴噺鎴愭姣旓紝鍥犳棰滆壊鐨勬繁娴呬笌缁嗚優鐨勫娈栨垚姝ｆ瘮锛屼笌缁嗚優姣掓€ф垚鍙嶆瘮銆備娇鐢ㄩ叾鏍囦华鍦?/span>450 nm娉㈤暱澶勬祴瀹欬/span>OD鍊硷紝鍙互鍙嶆槧娲荤粏鑳炴暟閲忋€侟/span>

CCK-8娉曚笌鏅€氱殑MTT娉曠浉姣旓紝鍏锋湁鏄捐憲鐗圭偣锛欬/span>

鈽匋/span>鐏垫晱搴﹂珮锛屾暟鎹彲闈狅紝閲嶇幇鎬уソ

鈽匋/span>鎿嶄綔绠€渚匡紝鐪佹椂鐪佸姏

鈽匋/span>姘存憾鎬э紝涓嶉渶瑕佹崲娑诧紝灏ゅ叾閫傚悎浜庢偓娴粏鑳濣/span>

鈽匋/span>鏃犻渶鏀惧皠鎬у悓浣嶇礌鍜屾湁鏈烘憾鍓傦紝瀵圭粏鑳炴瘨鎬т綆

鈽匋/span>涓裹/span>1鐡舵憾娑诧紝姣嬮渶棰勫埗锛屽嵆寮€鍗崇敤

鈽匋/span>閫傚悎浜庨珮閫氶噺鑽墿绛涢€堻/span>

CCK-8鐢ㄩ€旓細鑽墿绛涢€夈€佺粏鑳炲娈栨祴瀹氥€佺粏鑳炴瘨鎬ф祴瀹氥€佽偪鐦よ嵂鏁忚瘯楠孅/span>

CCK-8鏂规硶鐨勪紭鍔军/span>

CCK-8娉曚笌鍏朵粬缁嗚優澧炴畺/姣掓€ф娴嬫柟娉曠殑浼樺娍姣旇緝

妫€娴嬫柟娉旤/span>	MTT娉旤/span>	XTT娉旤/span>	WST-1娉旤/span>	CCK-8娉旤/span>
鐢茶嚋浜х墿鐨勬按婧舵€?/span>	宸紙闇€鍔犳湁鏈烘憾鍓傛憾瑙ｅ悗鍐嶆娴嬶級	濂键/span>	濂键/span>	濂键/span>
浜у搧鎬х姸	绮夋湯	2鐡舵憾娑秉/span>	婧舵恫	1鐡舵憾娑秉/span>
浣跨敤鏂规硶	閰嶆垚婧舵恫鍚庝娇鐢?/span>	鐜伴厤鐜扮敤	鍗冲紑鍗崇敤	鍗冲紑鍗崇敤
妫€娴嬬伒鏁忓害	楂楛/span>	寰堥珮	寰堥珮	楂楛/span>
妫€娴嬫椂闂滁/span>	杈冮暱	杈冪煭	杈冪煭	鏈€鐭?/span>
妫€娴嬫尝闀军/span>	560-600 nm	420-480 nm	420-480 nm	430-490 nm
缁嗚優姣掓€?/span>	楂橈紝缁嗚優褰㈡€佸畬鍏ㄦ秷澶包/span>	寰堜綆锛岀粏鑳炲舰鎬佷笉鍙楛/span>	寰堜綆锛岀粏鑳炲舰鎬佷笉鍙楛/span>	寰堜綆锛岀粏鑳炲舰鎬佷笉鍙楛/span>
璇曞墏绋冲畾鎬?/span>	涓€鑸?/span>	杈冨樊	涓€鑸?/span>	寰堝ソ
鎵归噺鏍峰搧妫€娴婞/span>	鍙互	闈炲父閫傚悎	闈炲父閫傚悎	闈炲父閫傚悎
渚挎嵎绋嬪害	涓€鑸?/span>	渚挎嵎	渚挎嵎	闈炲父渚挎嵎

淇濆瓨鏉′欢锛欬/span>

4oC閬垮厜淇濆瓨涓€骞存湁鏁堬紝-20oC閬垮厜淇濆瓨涓ゅ勾鏈夋晥銆侟/span>閬垮厤鍙嶅鍐昏瀺銆侟/span>

CCK-8璇曞墏鐩掓搷浣滆鏄嶞/span>

CCK-8婧舵恫鍙洿鎺ュ姞鍒扮粏鑳炰腑锛屼笉闇€瑕佷笌鍏跺畠缁勫垎棰勬贩銆傜敱浜嶞/span>CCK-8婧舵恫闈炲父绋冲畾骞朵笖缁嗚優姣掓€у緢灏忥紝鎵€浠ュ彲浠ヨ繘琛岄暱鏃堕棿瀛佃偛锛屼緥濡傚鑲秉/span>24-48灏忔椂銆侟/span>

鍦ㄨ繘琛屼互涓嬪疄楠屼箣鍓嶏紝鍙厛璁剧疆绌虹櫧瀵圭収瀛旓紝浠呭姞鍏ョ浉搴旈噺缁嗚優鍩瑰吇娑层€佽嵂鐗╁拰CCK-8婧舵恫锛屼笉鍔犲叆缁嗚優銆侟/span>

鏂规硶涓€.鍒朵綔鏍囧噯鏇茬嚎锛堟祴瀹氱粏鑳炲叿浣撴暟閲忥級

1銆佸厛鐢ㄧ粏鑳炶鏁版澘璁℃暟鎵€鍒跺鐨勭粏鑳炴偓娑蹭腑鐨勭粏鑳炴暟閲忥紝鐒跺悗鎺ョ缁嗚優鍒板煿鍏绘澘鍐呫€侟/span>

2銆佹寜姣斾緥锛堜緥濡傦細1/2姣斾緥锛変緷娆＄敤鍩瑰吇鍩虹瓑姣旂█閲婃垚涓€涓粏鑳炴祿搴︽搴︼紝涓€鑸鍋欬/span>3-5涓粏鑳炴祿搴︽搴︼紝姣忎釜娴撳害寤鸿3-6涓瀛斻€侟/span>

3銆佹帴绉嶅悗鍩瑰吇2-4灏忔椂浣跨粏鑳炶创澹侊紝鐒跺悗鎸夊煿鍏诲熀浣撶Н鐨凕/span>10%鍔犲叆CCK-8璇曞墏锛屽煿鍏讳竴瀹氭椂闂村悗娴嬪畾OD鍊硷紝鍒朵綔鍑轰竴鏉′互缁嗚優鏁伴噺涓烘í鍧愭爣锛圏/span>X杞达級锛孅/span>OD鍊间负绾靛潗鏍囷紙Y杞达級鐨勬爣鍑嗘洸绾裤€傛牴鎹鏍囧噯鏇茬嚎鍙互娴嬪畾鍑烘湭鐭ユ牱鍝佺殑缁嗚優鏁伴噺锛堜娇鐢ㄦ鏍囧噯鏇茬嚎鐨勫墠鎻愭槸瀹為獙鐨勬潯浠惰涓€鑷达紝渚夸簬纭畾缁嗚優鐨勬帴绉嶆暟閲忎互鍙婂姞鍏?/span>CCK-8鍚庣殑鍩瑰吇鏃堕棿銆傦級

鏂规硶浜孅/span>.缁嗚優娲绘€ф娴婞/span>

1銆佸湪96瀛旀澘涓帴绉嶇粏鑳炴偓娑诧紙100 渭L/瀛旓級銆傚皢鍩瑰吇鏉挎斁鍦ㄥ煿鍏荤涓鍩瑰吇涓€娈垫椂闂达紙37鈩傸/span>,5% CO2锛夈€侟/span>

2銆佸悜姣忓瓟鍔犲叆10 渭L CCK-8婧舵恫銆侟/span>

3銆佸皢鍩瑰吇鏉垮湪鍩瑰吇绠卞唴瀛佃偛1-4灏忔椂銆侟/span>

4銆佺敤閰舵爣浠祴瀹氬湪450 nm澶勭殑鍚稿厜搴︺€侟/span>

鏂规硶涓堻/span>.缁嗚優澧炴畺-姣掓€ф娴婞/span>

1銆佸湪96瀛旀澘涓姞鍏?/span>90 渭L鐨勭粏鑳炴偓娑层€傚皢鍩瑰吇鏉挎斁鍦ㄥ煿鍏荤棰勫煿鍏狐/span>24灏忔椂锛圏/span>37鈩傸/span>锛孅/span>5% CO2锛夈€侟/span>

2銆佸悜鍩瑰吇鏉垮姞鍏?/span>10 渭L涓嶅悓娴撳害鐨勫緟娴嬬墿璐ㄣ€侟/span>

3銆佸皢鍩瑰吇鏉垮湪鍩瑰吇绠变腑瀛佃偛閫傚綋鐨勬椂闂达紙渚嬪锛欬/span>6銆?/span>12銆?/span>24鎴朁/span>48灏忔椂锛夈€侟/span>

4銆佸悜姣忓瓟鍔犲叆10 渭L CCK-8婧舵恫銆侟/span>

5銆佸皢鍩瑰吇鏉垮湪鍩瑰吇绠卞唴瀛佃偛1-4灏忔椂銆侟/span>

6銆佺敤閰舵爣浠祴瀹氬湪450 nm澶勭殑鍚稿厜搴︺€侟/span>

娲诲姏璁＄畻锛欬/span>

缁嗚優娲诲姏(%)=[A(鍔犺嵂)-A(绌虹櫧)]/[A(涓嶅姞鑽?/span>)-A(绌虹櫧)] 脳100

A(鍔犺嵂)锛氬叿鏈夌粏鑳炪€?/span>CCK婧舵恫鍜岃嵂鐗╂憾娑茬殑瀛旂殑鍚稿厜搴?/span>

A(绌虹櫧)锛氬叿鏈夊煿鍏诲熀鍜孅/span>CCK婧舵恫鑰屾病鏈夌粏鑳炵殑瀛旂殑鍚稿厜搴?/span>

A(0鍔犺嵂)锛氬叿鏈夌粏鑳炪€?/span>CCK婧舵恫鑰屾病鏈夎嵂鐗╂憾娑茬殑瀛旂殑鍚稿厜搴?/span>

缁嗚優娲诲姏锛氱粏鑳炲娈栨椿鍔涙垨缁嗚優姣掓€ф椿鍔汓/span>

娉ㄦ剰浜嬮」:

1锛岀敱浜庝娇鐢?/span>96瀛旀澘杩涜妫€娴嬶紝濡傛灉缁嗚優鍩瑰吇鏃堕棿杈冮暱锛屼竴瀹氳娉ㄦ剰钂稿彂闂銆備竴鏂归潰锛岀敱浜嶞/span>96瀛旀澘鍛ㄥ洿涓€鍦堟渶瀹规槗钂稿彂锛屽彲浠ラ噰鍙栧純鐢ㄥ懆鍥翠竴鍦堢殑鍔炴硶锛屾敼鍔犵浉鍚岄噺鐨凕/span>PBS銆?/span>姘存垨鍩瑰吇娑诧紱鍙︿竴鏂归潰锛屽彲浠ユ妸96瀛旀澘缃簬闈犺繎鍩瑰吇绠卞唴姘存簮鐨勫湴鏂癸紝浠ョ紦瑙ｈ捀鍙戙€侟/span>

2锛屾湰璇曞墏鐩掔殑妫€娴嬩緷璧栦簬鑴辨阿閰跺偓鍖栫殑鍙嶅簲锛孅/span>鎵€浠ヨ繕鍘熷墏(渚嬪涓€浜涙姉姘у寲鍓侟/span>)浼氬共鎵版娴嬨€傚鏋滃緟娴嬬墿璐ㄦ湁姘у寲鎬ф垨杩樺師鎬х殑璇濓紝鍙湪鍔燑/span>CCK-8涔嬪墠鏇存崲鏂伴矞鍩瑰吇鍩猴紙闄ゅ幓鍩瑰吇鍩猴紝骞剁敤鍩瑰吇鍩烘礂娑ょ粏鑳炰袱娆★紝鐒跺悗鍔犲叆鏂扮殑鍩瑰吇鍩猴級锛屽幓鎺夎嵂鐗╁奖鍝嶃€傚綋鐒惰嵂鐗╁奖鍝嶆瘮杈冨皬鐨勬儏鍐典笅锛屽彲浠ヤ笉鏇存崲鍩瑰吇鍩猴紝鐩存帴鎵ｉ櫎鍩瑰吇鍩轰腑鍔犲叆鑽墿鍚庣殑绌虹櫧鍚告敹鍗冲彲銆傚煿鍏诲熀涓厷绾㈠拰琛€娓呯殑鍚稿厜搴︼紝鍦ㄨ绠楁椂閫氳繃鎵ｉ櫎绌虹櫧瀛斾腑鏈簳鐨勫惛鍏夊害鑰屾秷鍘伙紝鍥犳涓嶄細瀵规娴嬮€犳垚褰卞搷銆侟/span>

3锛屾湁鏉′欢鐨勬儏鍐典笅寤鸿閲囩敤澶氶€氶亾绉绘恫鍣紝鍙互鍑忓皯骞宠瀛旈棿鐨勫樊寮傘€傚姞鍏?/span>CCK-8璇曞墏鏃讹紝寤鸿鏂滆创鐫€鍩瑰吇鏉垮鍔狅紝涓嶈鎻掑埌鍩瑰吇鍩洪〉闈笅鍔狅紝瀹规槗浜х敓姘旀场锛屼細骞叉壈OD鍊艰鏁般€傚姞瀹屼箣鍚庢渶濂借兘澶熺蹇冿紝浠ュ厤CCK-8娑叉淮鎮寕鍦ㄥ涓娿€傜敤閰舵爣浠娴嬪墠闇€纭繚姣忎釜瀛斿唴娌℃湁姘旀场锛屽惁鍒欎細骞叉壈娴嬪畾銆侟/span>

4锛岃嫢鏆傛椂涓嶆祴瀹欬/span>OD鍊硷紝鍙互鍚戞瘡瀛斾腑鍔犲叆10 渭L 0.1M鐨凕/span>HCL婧舵恫鎴栬€匋/span>1% w/v SDS婧舵恫锛屽苟閬洊鍩瑰吇鏉块伩鍏変繚瀛樺湪瀹ゆ俯鏉′欢涓嬨€侟/span>24灏忔椂鍐呮祴瀹氾紝鍚稿厜搴︿笉浼氬彂鐢熷彉鍖栥€侟/span>

5锛屽缓璁厛鍋氬嚑涓瓟鎽哥储鎺ョ缁嗚優鐨勬暟閲忓拰鍔犲叆CCK-8璇曞墏鍚庣殑鍩瑰吇鏃堕棿銆傜櫧缁嗚優鍙兘闇€瑕佸煿鍏昏緝闀挎椂闂淬€傝嫢浣跨敤24瀛旀澘鎴朁/span>6瀛旀澘瀹為獙锛岃鍏堣绠楁瘡瀛旂浉搴旂殑鎺ョ閲忥紝骞舵寜鐓ф瘡瀛斿煿鍏诲熀鎬讳綋绉殑10%鍔犲叆CCK-8婧舵恫銆侟/span>

6锛岃嫢娌℃湁450nm鐨勬护鍏夌墖锛屽彲浣跨敤鍚稿厜搴﹀湪430-490 nm涔嬮棿鐨勬护鍏夌墖锛屼絾鏄?/span>450nm婊ゅ厜鐗囩殑妫€娴嬬伒鏁忓害鏈€楂樸€侟/span>

7锛岄噾灞炲CCK-8鏄捐壊鏈夊奖鍝嶃€傜粓娴撳害涓裹/span>1 mM鐨勬隘鍖栦簹閾呫€佹隘鍖栭搧銆?/span>CuSO4浼氭姂鍒迭/span>5%銆?/span>15%銆?/span>90%鐨勬樉鑹插弽搴旓紝浣跨伒鏁忓害闄嶇骇銆傚鏋滅粓娴撳害鏄?/span>10 mM鐨勮瘽锛屽皢浼欬/span>100%鎶戝埗銆侟/span>

8锛屾湰浜у搧浠呴檺浜庝笓涓氫汉鍛樼殑绉戝鐮旂┒鐢紝涓嶅緱鐢ㄤ簬涓村簥璇婃柇鎴栨不鐤楋紝涓嶅緱鐢ㄤ簬椋熷搧鎴栬嵂鍝併€備负浜嗘偍鐨勫畨鍏ㄥ拰鍋ュ悍锛岃绌垮疄楠屾湇骞舵埓涓€娆℃€ф墜濂楁搷浣溿€侟/span>